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Chromatography 2016
September 21-23, 2016
Volume 7, Issue 5(Suppl) J Chromatogr Sep Tech 2016
ISSN: 2157-7064 JCGST, an open access journal
conferenceseries .com
September 21-23, 2016 Amsterdam, Netherlands
World Congress on Chromatography
Feasibilityof correlatingseparationof ternarymixturesofneutral analytesviathinlayerchromatography
with supercritical fluid chromatography in support of green flash separations
Tony Q Yan1, M Ashraf-Khorassani2, A Akin1, F Riley1, C Aurigemma1 and L T Taylor1
1Pfizer Inc., USA
2Virginia Tech, USA
Methoddevelopment for flash liquid chromatography as normal phase and reversedphase traditionally employs preliminary
thin layer chromatography (TLC) with conventional solvents on bare silica. Extension to green flash chromatography via
correlation of TLC migration results with conventional polar/non-polar liquid mixtures and packed column supercritical fluid
chromatography (SFC) retention times via gradient elution on bare silica with a suite of carbon dioxide mobile phase modifiers
is reported. Feasibility of TLC/SFC correlation is individually described for eight ternary mixtures of a total of 24 neutral
analytes. The experimental criteria for TLC/SFC correlation was assumed to be as follows: SFC/UV/MS retention (tR) increases
among each of the three resolved mixture components; while, TLC migration (Rf) decreases among the same resolved mixture
components. Good correlations of all 24 analytes were observed via SFC on bare silica with methanol as the CO2 modifier and
TLC on bare silica with a methanol/dichloromethane (95/5) mixture.
Biography
Tony Q Yan is currently working for Pfizer, Inc. (Groton, CT, USA) in the field of impurity isolation for structure elucidation in the Department of Pharmaceutical
Science. He has been working in pharmaceutical research and development in the area of chiral and achiral purifications, and impurity isolation for over 20 years
since he graduated from the Department of Chemistry in University of Missouri in Rolla with PhD degree in 1995.
Tony Q Yan et al., J Chromatogr Sep Tech 2016, 7:5(Suppl)