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Chromatography 2016
September 21-23, 2016
Volume 7, Issue 5(Suppl) J Chromatogr Sep Tech 2016
ISSN: 2157-7064 JCGST, an open access journal
conferenceseries .com
September 21-23, 2016 Amsterdam, Netherlands
World Congress on Chromatography
Development of a multicomponent method for the analysis of banned substances in cosmetic products
by GC-MS/MS
Ševčík Václav1,2, Vavrouš Adam1,2, Čabala Radomír2,3, Vrbík Karel1
1National Institute of Public Health, Centre of toxicology and health safety, Czech Republic
2Charles University in Prague, Faculty of Science, Department of Analytical Chemistry, Czech Republic
3General University Hospital in Prague, Institute of Forensic Medicine and Toxicology, Czech Republic
Anew method based on solid phase extraction (SPE) followed by GC-MS has been developed for determination of 40
substances prohibited in the EU in water miscible matrices.The effect of several factors, including sorbent type, salt
addition, washing step and elution solvent, on the system response was tested using Taguchi experimental design approach
during optimization. Application of the statistical analysis on Taguchi’s signal to noise ratios helped to find the optimal values of
relevant factors for most compounds. In the optimized procedure, 60 mg of sample dissolved in water was directly extracted by
a/the preconditioned SPE column, eluted with 600 µL of Ethyl Acetate, and after dilution, the extract was analyzed by GC-MS/
MS without any further cleaning or concentration step. Accuracy, precision, detection limits and repeatability were evaluated
during method validation. To test the method reliability, analyte recoveries were determined on spiked real samples including
shampoos, shower gels, and face-wash gels. Resulting analyte recoveries varying from 40 to 110 %, repeatability (RSD) from
5 to 20 % and quantitation limits in µg/g range confirmed suitability of this method for routine testing of cosmetic products.
Biography
Autor graduated from Charles University in 2012. He has been working as analytical chemist in National Institute of Public Health since graduated and has been
studying PhD since 2013.
Ševčík Václav et al., J Chromatogr Sep Tech 2016, 7:5(Suppl)