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Chromatography 2016
September 21-23, 2016
Volume 7, Issue 5(Suppl) J Chromatogr Sep Tech 2016
ISSN: 2157-7064 JCGST, an open access journal
conferenceseries .com
September 21-23, 2016 Amsterdam, Netherlands
World Congress on Chromatography
New method of screening allergenic components from Yejuhua injection with LAD2/CMC model
online UHPLC-ESI-IT-TOF-MS system
Yanni Lv, Shengli Han and Langchong He
Xi’an Jiaotong University, China
Yejuhua (YJH) injection is a traditional Chinese medicine (TCM) extracted from the single herb Yejuhua (Chrysanthemi
Indici Flos, dry anthotaxy of Chrysanthemum indicum L.), which is widely used for the treatment of acute tonsillitis, upper
respiratory tract infection, and throat ache in clinical practice. Linarin, a flavonoid glucoside reported to be one of the major
active components of YJH injection, can cause inhibition of aldose reductase, phosphodiesterase and platelet aggregation. ADRs
caused by YJH injection, majorly manifested as allergic reactions. Non-IgE mediated drug hypersensitivities, also referred
to as pseudoallergic or anaphylactoid reactions have clinical manifestations that are often indistinguishable from allergic
reactions. Effective and practical method for allergen screening and identification in YJH injection is in need. Cell membrane
chromatography (CMC), developed by him and his colleague in 1996, is a new type of biological affinity chromatography and
has been confirmed as an effective method to screen bioactive components from complex systems. In the present study, an
LAD2/CMC-UHPLC-ESI-MS/MS method was established for screening, analyzing, and identifying the allergenic components
from YJH injection. A retained fraction on the LAD2/CMC column was got, and identified as linarin. In order to verify
whether linarin could induce LAD2 cells degranulation, histamine (HA) release assay was performed by the method of HPLC-
ESI-MS/MS we established before. Results showed that linarin had an allergic effect by increasing histamine release in a dose-
dependent manner from 10 to 100 µM. In conclusion, the LAD2/CMC-UHPLC-ESI-MS/MS system developed in this study
can be used to screen allergenic components in other TCM injections.
Biography
Yanni Lv is pursuing her PhD in School of Pharmacy at Xi’an Jiaotong University. She is pursuing her Doctor’s Degree in Pharmaceutical Analysis and supervised
by Professor Langchong He. She has completed her college studies from Xi’an Jiaotong University, a key university which is directly administered by the Chinese
Education Ministry and is one of the oldest current institutions of higher education in China.
Yanni Lv et al., J Chromatogr Sep Tech 2016, 7:5(Suppl)