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Chromatography 2016
September 21-23, 2016
Volume 7, Issue 5(Suppl) J Chromatogr Sep Tech 2016
ISSN: 2157-7064 JCGST, an open access journal
conferenceseries .com
September 21-23, 2016 Amsterdam, Netherlands
World Congress on Chromatography
Screening allergenic components from Danshen injection via HMC-1/CMC online UHPLC-ESI-MS/
MS system
Yuanyuan Lin, Meng Sun, Tao Zhang, Delu Che and Langchong He
Xi’an Jiaotong University, China
Danshen injection is a Traditional Chinese Medicine (TCM) injection widely used in China to treat coronary heart disease
and angina. Adverse drug reactions of danshen injection, majorly manifested as allergic reactions, were among the leading
causes of death fromTCM injection. In the present study, an HMC-1/CMC online UHPLC-ESI-MS/MS systemwas established
to screen and identify allergenic components in danshen injection, by which salvianolic acid A, isosalvianolic acid C, and
salvianolic acid C were identified as potential allergenic components. Allergenic activities of salvianolic acid A, isosalvianolic
acid C, and salvianolic acid C were investigated in HMC-1 cell intracellular Ca2+ mobilization assay, histamine release and
β-hexosaminidase release tests in vitro. The results showed that the changes in Ca2+ influx in HMC-1 cell clearly increased
under salvianolic acid A (100 μM), isosalvianolic acid C (12.5 μM) and salvianolic acid C (25 μM) treatment respectively. In
addition, β-hexosaminidase and histamine release in HMC-1 cell were both markedly enhanced with increased concentrations
of salvianolic acid A, isosalvianolic acid C, and salvianolic acid C. The HMC-1/CMC online UHPLC-ESI-MS/MS system
developed in this study is an effective method for screening and identifying allergenic components from danshen injection,
and it may potentially be used to screen allergenic components in other TCM injections.
Biography
Yuanyuan Lin is pursuing her PhD from School of Medicine, Xi’an Jiaotong University. She completed her graduation from South-Central University for nationalities
in 2013. Her research interests are “Development of advanced analysis methods for rapid, accurate and high throughput screening target compounds from the
complex samples such as Traditional Chinese Medicine injection”. She is the Co-author of three original research papers published in international journals.
Yuanyuan Lin et al., J Chromatogr Sep Tech 2016, 7:5(Suppl)