chromatography-2016_scientifictracks-abstracts

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Chromatography 2016

September 21-23, 2016

Volume 7, Issue 5(Suppl)

J Chromatogr Sep Tech 2016

ISSN: 2157-7064 JCGST, an open access journal

conferenceseries

.com

September 21-23, 2016 Amsterdam, Netherlands

World Congress on

Chromatography

New applications of reversed phase chromatography for detection and quantitation of certain adulterants

in illegal herbal medicines

Ahmed Mohamed Ali Hemdan

and Shereen M Tawakol

Ahram Canadian University, Egypt

Helwan University, Egypt

reversed phase HPLC method with UV detection has been developed and subsequently validated for the determination of

some adulterants in illegal herbal medicines. Five herbal medicines for weight loss and other one for erectile dysfunction

were tested for the presence of possible adulterants.The weight loss products were found adulterated with the already withdrawn

drug Sibutramine and with Phenolphthalein that is proved to cause tumors. On the other hand, Sildenafil, a contraindicated

drug for patients with heart diseases, was found in the herbal products for erectile dysfunctions. The methods use Inertsil C18

column (250x4.6 mm, 5 µ) and a flow rate of 1 mL/min. The mobile phase for quantitation of Sibutramine and Phenolphthalein

was consisting of acetonitrile-potassium hydrogen phosphate buffer pH=3 adjusted by o-phosphoric acid (40/60 v/v) and

detection at 223 nm. While the mobile phase for Sildenafil was acetonitrile-potassium hydrogen phosphate buffer pH=3.2

adjusted by o-phosphoric acid (50/50 v/v) and detection at 230 nm. The confirmation of the presence of the adulterants was

done using LC-PDA andMASS spectrometry. For this, a complete investigation of any herbal medicine should be done through

orthogonal analysis methods before use even if it is labeled with (100% herbal content).

Biography

Ahmed Mohamed Ali Hemdan has completed his PhD from Ain Shams University. He has published more 10 papers in reputed journals.

Ahmed Mohamed Ali Hemdan et al., J Chromatogr Sep Tech 2016, 7:5(Suppl)