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Volume 6 Journal of Neurological Disorders
ISSN: 2329-6895 CNS 2018
November 12-13, 2018
November 12-13, 2018 | Edinburgh, Scotland
4th International Conference on
Central Nervous System Disorders & Therapeutics
Beneficial effect of oral supplementation of virgin coconut oil on methotrexate induced cerebral
neurotoxicity and inflammation in rats
Ademola C Famurewa1, Patrick M Aja2, Joshua N Awoke2, Ekenechukwu K Maduagwuna 2, Chinyere Aloke1 and Nwatu U Ezekiel2
1Alex Ekwueme Federal University Ndufu-Alike, Nigeria
2Ebonyi State University, Nigeria
Background: Methotrexate is an anticancer drug associated with several toxicities on organs through oxidative stress and
inflammation. The study evaluated oral supplementation of virgin coconut oil (VCO) against cerebral neurotoxicity and
inflammation induced by methotrexate (MTX) in rats.
Methods: Eighteen Wistar rats were divided into three groups (n=6) as Control, MTX and VCO + MTX. Rats were orally
administered VCO (5 mL/kg) for 17 consecutive days, while a single dose of MTX (20 mg/kg bw, i.p.) was administered on
day 14 only. Homogenate samples obtained from the cerebrum were used to analyze antioxidant enzymes, reduced glutathione
(GSH), malondialdehyde (MDA), nitric oxide (NO), acetylcholinesterase (AchE) and inflammatory cytokines tumor necrosis
factor-α (TNF-α) and interleukin-6 (IL-6).
Results: MTX administration significantly (p<0.05) reduced activities of antioxidant enzymes and level of GSH in cerebrum,
whereas MDA, NO, AchE, TNF-α and IL-6 levels significantly (p<0.05) increased in cerebrum relative to normal control. In
contrast, oral supplementation of VCO attenuated the MTX induced biochemical alterations relative to MTX group in the
current study.
Conclusion: Our findings suggest beneficial effect of VCO against MTX neurotoxicity. Oral supplementation of VCO may
prevent MTX neurotoxicity in cancer patients.
J Neurol Disord 2018, Volume 6
DOI: 10.4172/2329-6895-C9-050