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Euro Biotechnology 2016
November 07-09, 2016
Volume 6, Issue 7(Suppl) J Biotechnol Biomater
ISSN: 2155-952X JBTBM, an open access journal
conferenceseries .com
November 07-09, 2016 Alicante, Spain
12th Euro Biotechnology Congress
Ileana Vera Reyes et al., J Biotechnol Biomater 2016, 6:7(Suppl)
Utilization of zinc oxide nanoparticles doped with silver against phytopathogenic fungi
Ileana Vera Reyes1, Lira-Saldivar R H1, Esparza-Arredondo I J E2, Mendez-Arguello B1, Mendoza-Mendoza E1 and Garcia-Cerda L A 1
1Centro de Investigacion en Química Aplicada, Mexico
2Universidad Autonoma Agraria Antonio Narro, Mexico
Modern agriculture requires low impact products; metallic nanoparticles (NPs) with antimicrobial effects could serve for
this purpose. For that reason the aimof this work was to analyze the effect of pure zinc oxide nanoparticles (ZnONPs) and
zinc oxide NPs doped with silver (1.25 and 2.5 %w/w), against two very damaging plant pathogenic fungi: Fusarium oxysporum
and Alternaria solani, which are the main pathogens responsible for severe diseases of a large number of agricultural crops.
We synthesized nanometric ZnO particles at very low-temperature by using a mechanically assisted metathesis reaction, that
permitted the formation of spherical nanoparticles with mean sizes of around 20 nm. NPs characterization was accomplished
by X-ray diffraction; the size and shape of the particles were studied by transmission electron microscopy (TEM). The effect
of ZnO NPs and Ag-doped ZnO NPs against the fungi was done using NPs incorporated to PDA growth medium at different
doses (0, 250, 500 and 1000 mg L-1). ANOVA and Tukey multiple range tests were used to analyze data. The application of 1000
mg L-1 concentration of ZnO NPs produced maximum growth inhibition of fungal hyphae. We also assessed the effectiveness
of combining the ZnO and silver nanoparticles, however they did not exhibit a greater antimicrobial activity than pure ZnO
NPs. Based on this results; it is viable that tested ZnO NPs could be used in programs of sustainable agriculture, since they are
required in minute quantities by comparison to conventional pesticides.
Biography
Ileana Vera Reyes has completed her PhD from Centro de Investigacion en Estudios Avanzados del Instituto Politecnico Nacional, Mexico. She is a recipient of a
CONACYT Research Fellow.