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Volume 8 Journal of Gastrointestinal & Digestive System Gastro Meet 2018
August 06-07, 2018
August 06-07, 2018 Abu Dhabi, UAE
Gastroenterology and Digestive
Disorders
16th International Conference on
Study of the proteomic profile in patients with Crohn's disease, its correlation with diagnosis and
disease activity
Ezzat Ali Ahmed1, Doaa Abdou Mohamed Header1, Salah El-Din Ahmed Badr El-Din1, Pacint El-Saed Moez2, Mohamed Eid Ibrahim3
1Departments of Internal Medicine
2Clinical Pathology, Radiodiagnosis
3Faculty of Medicine, Alexandria University, Egypt
Background: Inflammatory bowel diseases (IBD) include two major forms of chronic intestinal disorders: Crohn’s disease (CD)
and ulcerative colitis (UC). CD can be associated with intestinal granulomas, strictures, fistulas, and transmural inflammation. A single
gold standard for the diagnosis of CD is not available.The diagnosis is confirmed by clinical evaluation and a combination of endoscopic,
histological, radiological, and/or biochemical investigations. Serum protein profiling of CD was investigated in order to improve the
comprehension of the pathologicmechanisms and to support the difficult diagnostic procedures currently available.The aim of the work is to
identify plasma proteomic profiles of CD cases and correlating this profile with the other diagnostic markers and activity of the disease.
Methods: We performed a study with 64 plasma samples collected from patients classified in 2 groups (31 crohn's disease, 33
healthy controls) according to accredited criteria. They were subjected to: complete history taking, thorough clinical examination,
Laboratory investigations (Erythrocyte sedimentation rate ESR, C- reactive protein CPP, fecal calprotectin, Anti-Saccharomyces
cerevisiae antibodies ASCA), ileocolonscopy, histopathology, imaging were done. Plasma proteomic pattern of CD patients
and control subjects was determined using Matrix-Assisted Laser Desorption/Ionization (MALDI) Time of Flight (TOF) Mass
Spectrometer (MS) analysis, all plasma samples were subjected to solid-phase extraction (SPE). The spectra obtained from all the
samples were analysed using ClinProTool software.
Results: There was a statistical significant difference of the plasma proteome profiles of CD group in comparison to health
volunteers. 76 peptide peaks were identified by the ClinProt software with a statistically different area, 5 peptide peaks were highly
significant. Sensitivity was found to be 91.7%, specificity was 78.6%, PPV was 90, NPV was 77.5 and Youden Index was 0.88.
There was a statistical significant difference between active versus inactive CD group, 5 Integration Regions used for classification
between active and inactive CD patients using Genetic Algorithm model (GA) which gave 81.43% cross validation and 100%
recognition capability. Markers as ESR, CRP, fecal calprotectin, ASCA are statistically correlated to the plasma proteomics found
in CD patients. Conclusion: Proteomic profile has the potential to improve diagnosis and evaluate CD activity, reducing the need
for more invasive techniques. Plasma proteome profile of CD was statistically correlated to other markers.
Keywords: Proteomics; CD; Biomarkers; (MALDI) TOF MS; Inflammation; Serum profiling
J Gastrointest Dig Syst 2018, Volume 8
DOI: 10.4172/2161-069X-C3-071
Pediatric Endoscopy, Every day a challenge for the Pediatric Gastroenterologist
Aldana Grisales Alexandra
Spain
As other Medical Thecniques, pediatrics follow adults and adapt their advances in our practice. It is like, Flexible endoscopy
is used since beguinning of XX century per Basil Hirschowitz, and pediatric endoscopy (PE) is used just since 1970 The aim
of this work is making a literature review about the all issues aroud PE Pediatric GE should preform most of the PE procedure
as diagnosis and treatment. Just more advanced procedures as ERCP and EUS must be preformed by Adult endoscopist with
especial training Esophagogastroscopy indicated in allergic, infectus and peptic esophagitis, Infectus and inflamatory gastritis
and coeliac disease Colonoscopy diagnosis in IBD, rectal bleeding and Infantile Polyposis. No routinary screening of cancer as
adult, but special consideratiosn in surveillance in longterm IBD and juvenile Polyposis ERCP indicated in Choledocolithiasis,
Primary Sclerosis cholangitis biliary strictures or lake after liver transplantation. pancreatic ERCP in pancreas divisum or chonic
pancreatitis It can be donde since 10kg weight children EUS Indicated in pancreatic disorders and rectal malformation and
fistulizing IBD In patients since 10 kg weight Endoscopic Capsula Aproved in older than 2 years old in ocult bleeding, coeliac
disease and Crohn Disease characterization Single Or Double Ballon Enterocopy indicated in small bowel involment in Pediatric
Crohn, strictures or vascular lesions. Or assisted to surgery It can be done since 10 kg of weight.