10 / 12
Page 32
conferenceseries
.com
Volume 8
Journal of Gastrointestinal & Digestive System
Gastroenterology Congress 2018
August 06-07, 2018
August 06-07, 2018 Osaka, Japan
14
th
Annual Congress on
Gastroenterology & Hepatology
J Gastrointest Dig Syst 2018, Volume 8
DOI: 10.4172/2161-069X-C4-074
Growth, acid production, bile tolerance and adherence to columnar epithelial cells of four species of
bifidobacteria
Ahmed Zahran
Minia University, Egypt
F
our species of bifidobacteria were studied for growth, acid production; bile acids tolerance and adherence to sheep epithelial
cells were studied. The species examined were
Bifidobacterium longum
ATCC 15707,
B. bifidum
ATCC 2203,
B. angulatum
ATCC 2238 and
B. breve
ATCC 2258 were tested for growth and environmental conditions. The log phase of
B.longum
and
B.angulatum
were found to be after 14 hours phase whereas the log phase of the other two species were found to be after 17
hours of inoculation. Both
B. longum
and
B. angulatum
reduced the pH faster than both
B. bifidum
and
B. breve
.
B. longum
had
the highest growth and
B. breve
had the lowest growth rate. All the studied species exhibited some degree of bile tolerance.
B.
longum
and
B. bifium
were more resistant to bile acids than the other two species. Adhesion of the four species to the columnar
epithelial cells of the small intestine of sheep was studied. All the tested species showed some degree of adhesion; however,
B.
longum
adhered to the epithelial cells more than other three species.
asz52@yahoo.co.uk