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Volume 8

Journal of Gastrointestinal & Digestive System

Gastroenterology Congress 2018

August 06-07, 2018

August 06-07, 2018 Osaka, Japan

Annual Congress on

Gastroenterology & Hepatology

Expression of the inflammatory regulatorA20 correlates with disease activity in ulcerative colitis patients

Jaishree Paul

, Ishani Majumdar

and Vineet Ahuja

Jawaharlal Nehru University, India

All India Institute of Medical Sciences, India

n aberrant immune response arising from intolerance towards luminal antigens and commensal microflora is a hallmark

of UC. Transcription factor NF-кB is a major regulatory component influencing mucosal inflammation during disease

condition. A20

(TNFAIP3-

tumor necrosis factor alpha-induced protein 3 is an endogenous negative regulator of the NF-кB

cascade. A20 forms a ubiquitin editing complex with the association of its partner molecules-

ITCH, RNF11

and

Tax1BP1

for

its activation. Here, we have explored the expression of the above molecules in inflamed mucosa of UC patients vs. controls.

mRNA expression of A20 significantly up regulated but negatively correlated with disease activity. However, mRNA levels of

ITCH, RNF11

and

Tax1BP1

significantly down-regulated with the severity of disease. Interestingly, all the four genes exhibited

significant down regulation at protein level in patient’s samples. NF-кB binding activity was traced to mRNA expression of the

p65-subunit of NF-кB and

MAST3

. We observed significant increase in p65 mRNA expression and down-regulated

MAST3

expression. These observations suggested that A20 level is regulated by increase in expression of NF-κB. Down-regulation

of A20 observed in patient samples correlated with the significant up-regulation of p65 sub-unit of NF-κB. This was further

validated with

iNOS

gene, an inflammatory marker and with inhibitors of apoptosis, cIAP2 and XIAP as well as mediators of

anti-apoptotic signals TRAF1 and TRAF2. We conclude that decreased expression of A20 and its partner molecules contribute

to inflammation process by up-regulating the expression of NF-κB and further confirmed that up-regulation of apoptosis

inhibitors probably create micro-environment for colorectal cancer.

J Gastrointest Dig Syst 2018, Volume 8

DOI: 10.4172/2161-069X-C4-074