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.com
Volume 5, Issue 3 (Suppl)
Mod Chem Appl, an open access journal
ISSN: 2329-6798
Global Chemistry 2017
September 04-06, 2017
September 04-06, 2017 | London, UK
5
th
Global Chemistry Congress
Photochemistry, isolations and some pharmacological studies of ethanol leaf extract of
Piliostigma thonningii
Fanna Inna Abdulrahman
University of Maiduguri, Nigeria
T
his study was aimed at evaluation of phytochemical constituents and the effect of ethanol leaf extract of
Piliostigma thonningii
on
the central and peripheral nervous systems in laboratory animals. Fresh leaves of
Piliostigma thonningii
were air-dried, pulverized
extracted using soxhlet extraction technique with ethanol 148.24% w/w after being concentrated. The extract was screened for
phytochemicals using standard methods. 20 g of the ethanol extract was subjected to column chromatographic (CC) analysis using
ethyl acetate and n-butanol as mobile phase at different ratios and silica gel of 60-120 mesh as the stationary phase. Fractions obtained
with similar retention factor (R
f
) using thin layer chromatography (TLC) were combined, coded and subsequently screened for
phytochemicals. Subsequent purification of fraction PTE3 was carried out using CC (ethyl acetate and methanol were used as mobile
phase at different ratios) and TLC until a sub-fraction PTE34 among other fractions gave a single spot on TLC and had a melting
point of 102-103oC. The phytochemical studies of the ethanol leaf extract of
Piliostigma thonningii
revealed the presence of some
useful chemical compounds such as flavonoids, cardiac glycosides, tannins, saponins, and terpenoids. The pharmacological effects
of
Piliostigma thonningii
was determined by examining the effects of the leaf extract on phenobarbitone sleeping time, analgesic and
muscle relaxant activities using experimental animals. The analgesic effect of the leaf extract was evaluated with acetic acid induced
writhing and thermally induced nociception for pain. It was observed that the extract conferred 48.00 and 57.20% protection from
writhes induced by acetic acid on mice when extract doses of 200 and 400 mg/Kg were administered. Similarly, there was a significant
(p<0.5) dose dependent effect conferred on mice when pain was induced by heat. The extract also had a muscle relaxant effect as 20%,
60% and 80% were observed to slide down an inclined board in a dose dependent manner. The extract also significantly potentiated
sleeping time of phenobarbitone dose dependently in rats of which the mean time duration of (72.0±04.64) min, (83.40±02.11) min,
and (123.60±11.57) min were observed when rats were administered extract doses of 200, 400 and 600 mg/Kg b wt. Thus, the ethanol
leaf extract of
Piliostigma thonningii
was able to provide depressant effects which were shown in its ability to potentiate barbiturate
sleeping, analgesia and muscle relaxant effect.
Biography
Fanna Inna Abdulrahman is presently a Lecturer of Organic Chemistry in Department of Chemistry, University of Maiduguri. She completed her PhD in Medicinal
Chemistry in 1997. She has published more than 100 research papers in the field of Organic/Medicinal Chemistry and Natural Product. Her current research interest
is in the area of Organic/ Medicinal Chemistry and Natural product. She is a Fellow of Chemical Society of Nigeria, Fellow of Institute of Chartered Chemist of
Nigeria, Member of West African Network of Natural Products Research Science and Member of Council of the Institute of Chartered Chemist of Nigeria. She was
the Head of Department of Chemistry, University of Maiduguri, Borno State, Nigeria.
fannas7@yahoo.comFanna Inna Abdulrahman, Mod Chem Appl 2017, 5:3(Suppl)
DOI: 10.4172/2329-6798-C1-005