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Volume 5, Issue 3 (Suppl) Mod Chem Appl, an open access journal
ISSN: 2329-6798 Global Chemistry 2017
September 04-06, 2017
September 04-06, 2017 | London, UK
5th Global Chemistry Congress
Nanofiber mats fabricated by solution blow spinning as potential substrates for cardiac cell culture
Ewelina Tomecka, Michal Wojasinski, Elzbieta Jastrzebska, Michal Chudy, Tomasz Ciach and Zbigniew Brzozka
Warsaw University of Technology, Poland
In recent years, there is a growing interest in the use of nanofibers for cell culture. Nano fibrous materials have many advantages
such as: high porosity, high surface to volume ratio and they are structurally similar to extracellular matrix (ECM). Furthermore,
nanofibers structure influence on cell orientation, which allow mimicking natural organization of cardiac cells. Most researches use
electrospinning for nanofibers fabrication. However, low efficiency of this technique doesn’t allow to use it for large-scale production
of nanofibers. We propose a rarely used method - solution blow spinning (SBS), which allows scale-up the nanofibers manufacturing
process to the commercial level. This work presents the comparison and evaluation of cardiac cell proliferation on poly-(L-lactic
acid) (PLLA) and polyurethane (PU) nano-fibrous mats fabricated by SBS. For the experiments, three different cardiac cell lines were
used (figure 1). Cell cultures were performed for seven days on non-modified and protein-modified nanofibers surface. Obtained
results of cardiac cell culture on investigated surfaces of nanofibers were compared to results of cardiac cell culture on polystyrene
(PS) surfaces modified in the same way. The results showed the all types of investigated cells cultured on nanofibers (PLLA and PU)
have more elongated shape than cells cultured on PS surface. Moreover, cells were arranged in parallel to each other, according to
fibers orientation. In contrast, cells on PS surfaces were oriented randomly. Furthermore, in most cases, the cells proliferated better
on nanofibers (PLLA and PU) than on PS surfaces modified in the same way. The results indicated that polymeric nanofibers (PLLA
and PU) are better substrates for cardiac cell culture than PS surface and they enable cultivating these cells with conditions more
similar to in vivo environment.
Biography
Ewelina Tomecka received her MSc in Biotechnology from Warsaw University of Technology in 2011. Now, she is a PhD student at Institute of Biotechnology (WUT)
and a member of Chemical Sensors Research Group. Her current research interests include “Development of a microfluidic cardiac cell culture model”.
Ewelina Tomecka et al., Mod Chem Appl 2017, 5:3(Suppl)
DOI: 10.4172/2329-6798-C1-005