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Volume 7, Issue 4 (Suppl)
J Clin Trial
ISSN: 2167-0870 JCTR, an open access journal
Global Pharmacovigilance 2017
July 06-07, 2017
JULY 06-07, 2017 KUALA LUMPUR, MALAYSIA
8
TH
GLOBAL
Pharmacovigilance &
Drug Safety Summit
Gastric microbiome and ovine microhabitat variations in response to early and late stages of
haemonchus contortus infection
Saeed El-Ashram
Kafr El-Sheikh University, Egypt
T
he interactions between gastric microbiota, ovine host, and
Haemonchus contortus
portray the ovine stomach environment
as a complex ecosystem, where all factors play a pertinent role in fine-tuning each other and in the homeostaticmaintenance.
We delineated the impact of early and late
Haemonchus
infection on abomasal and ruminal microbial community, and ovine
host. Twelve parasite-naive lambs were divided into four treatments, uninfected-control groups and 7- and 50- day post-
infected groups, in triplicate. Six sheep were inoculated with 5,000 of
H. contortus
infective larvae and followed for 7 and 50
days with their corresponding uninfected-control ones. Ovine stomach tissues were collected for histological and anatomical
analyses and gastric fluids collected to measure PH values, microbial community isolated and subjected to the Illumina
MiSeq platform and bioinformatic analysis. Our results showed that
Haemonchus
infection increased the abomasal gastric
pH (P=0.04953) and caused a substantial augmentation in anterior blind sac papillae numbers (P=0.0463), as well as resulted
in necrotizing and inflammatory changes that were more severe during acute infection. Furthermore, infection increased
the abomasal bacterial load and decreased the ruminal microbiome, but abrogated Archea in both gastric compartments. A
7-day infection of sheep with
H. contortus
significantly altered approximately 98% and 94% of genera in the abomasal and
ruminal bacterial profile, respectively (P=0.0369-0.0495). However, the approximate altered genera 50 days after infection in
the ovine abomasal and ruminal microbiome were about 62% and 69%, correspondingly (P=0.0369-0.0495) with increase in
some bacteria and decrease in others. Overall, these results indicate that
Haemonchus
infection plays a crucial role for shaping
stomach microbial community composition, and diversity.
saeed_elashram@yahoo.comJ Clin Trial 2017, 7:4 (Suppl)
DOI: 10.4172/2167-0870-C1-017