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.com
Volume 7, Issue 4 (Suppl)
J Clin Trial
ISSN: 2167-0870 JCTR, an open access journal
Global Pharmacovigilance 2017
July 06-07, 2017
JULY 06-07, 2017 KUALA LUMPUR, MALAYSIA
8
TH
GLOBAL
Pharmacovigilance &
Drug Safety Summit
Preparation and tumor-suppressing effects of folic acid-targeted temperature-sensitive magnetoliposomes in
ovarian cancer
Xi Hui Wang
1
, Qiusha Tang
1
, Yan Li An
2
, Chunyan Yuan
2
and Daozhen Chen
3
1
Southeast University, China
2
Zhongda Hospital, China
3
Wuxi Maternal and Child Health Hospital, China
Background & Aim:
Ovarian cancer is a tumor with a poor prognosis. It is a common gynecological malignancy and because
of the heat sensitivity and the unique nature of ovarian cancer development, the development of effective anti-ovarian cancer
techniques involving hyperthermia may have clinical value. We prepared folate-targeted thermosensitive liposomes wrapped
with 17-AAG chemotherapy drugs and superparamagnetic material (17-AAG/MTSLs-FA) and assessed combined magnetic
fluid hyperthermia with ovarian cancer SKOV3 cells
in vitro
and
in vivo
.
Methods:
The magnetic thermosensitive liposomes wrapped with 17-AAG chemotherapy drugs and chemically co-precipitated
Fe3O4 magnetic nanoparticles were prepared by a rotary evaporation method. The liposome morphology and distribution was
observed using TEM, particle size and zeta potential were measured by a dynamic light scattering method, drug encapsulation
efficiency was detected by UV spectrophotometry and the temperature- controlled release properties were determined by a
dialysis method. Experiments were conducted using the SKOV3 human ovarian cancer cell line and MCF7 human breast
carcinoma cells to evaluate anti-tumor effects.
Results:
17-AAG/MTSLs-FA prepared in this study met the basic requirements; the preparation method is simple and the raw
materials are readily available. The product exhibited strong magnetic and high encapsulation efficiencies, good performance
and low toxicity. The liposomes combined with hyperthermia inhibited the proliferation of human ovarian cancer SKOV3 cells
significantly and induced apoptosis. There was an intergroup difference in doubling time
in vitro
and
in vivo
(P<0.05).
Conclusions:
Folic acid-conjugated 17-AAGmagnetic thermosensitive liposomes in combination with an alternating magnetic
field for heating can achieve a combined synergistic anti-tumor effect of the chemotherapy and heat treatment, potentially
offering a new method for ovarian cancer treatment.
wxhwcc@126.comXi Hui Wang et al., J Clin Trial 2017, 7:4 (Suppl)
DOI: 10.4172/2167-0870-C1-017