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April 16-17, 2018 Las Vegas, Nevada, USA
2
nd
Edition of
Graphene & Semiconductors | Diamond Graphite & Carbon Materials Conference
6
th
Edition of
Smart Materials & Structures Conference
&
Journal of Material Sciences and Engineering| ISSN: 2169-0022 | GDCSM-2018 | Volume: 7
A rapid and sensitive detection of glutathione using nanostructured V
2
O
5
mimicking the oxidase activity
as inorganic nanozyme
Akhilesh Babu Ganganboina
1
and
Ruey-an Doong
1,2
1
National Tsing Hua University, Taiwan
2
National Chiao Tung University, Taiwan
G
lutathione detection has been one of the important and critical line of investigations for biotechnologists in recent years. Herein,
we introduce vanadium pentoxide nanosheets (V
2
O
5
NS) as a novel nanozyme mimicking peroxidase reaction as a fast selective
colorimetric assay for the detection and quantification of glutathione (GSH). The V
2
O
5
NS have been prepared by ultrasonication
assisted exfoliation of bulk V
2
O
5
and characterised. The present process involves catalysis by V
2
O
5
in the oxidation of pale yellow
3,3ʹ,5,5ʹ,-tetramethylbenzidine (TMB) to blue color with an absorption peak centered at 650 nm. On introduction of GSH, a fading in
deep blue color of oxidized TMB occurs with a simultaneous decrease in absorbance intensity at 650 nm, indicating the sensitivity of
V
2
O
5
catalysed reaction. Also, GSH selectively inhibits this reaction with a detection limit of 10 nM. The high specificity of inhibition
by GSH allows this system to be used for determination of GSH concentration in human serum samples. This method is simple, fast
and cost effective and can evolve as a potential method in discriminatively detecting GSH.
ganganboinaakhilesh89@gmail.comJ Material Sci Eng 2018, Volume: 7
DOI: 10.4172/2169-0022-C3-098