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Page 60
conferenceseries.com
June 26-27, 2017 San Diego, USA
13th International conference on
Pathology and Molecular Diagnosis
Volume 7, Issue 2 (Suppl) J Clin Exp Pathol, an open access journal
ISSN:2161-0681
Pathology and Molecular Diagnosis 2017
June 26-27, 2017
J Clin Exp Pathol 2017, 7:2 (Suppl)
DOI: 10.4172/2161-0681-C1-035
Sputum but not blood periostin levels correlate with sputum eosinophil counts in asthma
Marie-Chantal Larose, Anne-Sophie Archambault, Véronique Provost, Jamila Chakir, Michel Laviolette and Nicolas Flamand
Université Laval, Canada
Objective: Periostin is increasingly recognized as a biomarker, notably in atopic dermatitis. Recent studies underscored that
serum periostin was one of the best biomarkers of severe asthmatics with persistent airway eosinophils and symptomatically
uncontrolled despite high doses of corticosteroids. However, given that the role of periostin to identify eosinophilic asthma is
still debated, we evaluated whether blood and sputum periostin levels link to sputum eosinophil counts and asthma severity.
Methods & Measurements: Blood and induced sputum samples were obtained from healthy and asthmatic (mild, moderate,
severe non-eosinophilic, severe eosinophilic) subjects, and blood samples from atopic dermatitis subjects. Human bronchial
epithelial cells (BECs) obtained from healthy subjects and mild or severe eosinophilic asthmatics were treated with IL-13.
Periostin and CCL26 levels were quantitated by ELISA.
Main Results: In sputum, eosinophil counts, CCL26 and periostin levels significantly correlated. Plasma periostin levels were
similar in healthy and asthmatic subjects. In asthmatics, they correlated with sputum eosinophil counts in severe eosinophilic
asthma, but weakly in the whole group. Of note, BECs of severe eosinophilic asthmatics released greater amounts of periostin
than those of mild asthmatics and healthy subjects. Blood eosinophil counts did not correlate with plasma periostin levels and
showed a slightly better correlation with sputum eosinophil counts.
Conclusions: Except for severe eosinophilic asthma, plasma periostin levels weakly correlated with sputum eosinophil counts
and could not predict the degree of bronchial eosinophilic inflammation. Both sputum periostin and CCL26 levels showed
better correlation with sputum eosinophil counts.
Biography
Marie-Chantal Larose has completed her Master’s from Laval University. She is completing her Doctoral studies at the Laval University, under the supervision of
Dr. Michel Laviolette and Dr. Nicolas Flamand.