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conferenceseries

.com

June 26-27, 2017 San Diego, USA

International conference on

Pathology and Molecular Diagnosis

Volume 7, Issue 2 (Suppl)

J Clin Exp Pathol, an open access journal

ISSN:2161-0681

Pathology and Molecular Diagnosis 2017

June 26-27, 2017

Impact of substance use on salivary cytokine levels in healthy female individuals

Miranda Li, Hazel Huang

and

Anna-Barbara Moscicki

University of California, USA

ubstance (tobacco, alcohol) use and various inflammatory diseases (periodontitis, oral lichen planus, leukoplakia) induce

chronic inflammation, which is a mechanism for head and neck squamous cell carcinoma (HNSCC). Analysis of salivary

cytokine levels reveals abnormal cytokine production, which, if detected early enough, could improve treatment and survival

rates for HNSCC. The correlation between substance use and cytokine levels has not been well researched. This pilot study

examines the correlation between substance use (tobacco, alcohol, marijuana) and cytokine levels (IFN-α, IL-10, IL-12, IL-

13, MIP-1α, TNF-α, IL-4, IL-6, IL-8, IL-1α, IL-1β) in 71 healthy women (25-32 years old). Luminex-based multi-analyte

MILLIPLEX™ MAP Human Cytokine/Chemokine Magnetic Bead Kits (Millipore Corp., Billerica, MA) andMAGPIX® imaging

technology was used to analyze the saliva samples. There was a statistically significant difference in cytokine interleukin (IL)-

1β levels between the control group (n=24, SD=26.90) and the tobacco/light alcohol user group (n=21, SD=164.14), p≤0.05.

There was also a statistically significant difference in cytokine IL-8 levels between the control group (n=24, SD=236.58) and

the tobacco/heavy alcohol user group (n=18, SD=295.26), p≤0.01. These results suggest that young women who use tobacco

and alcohol heavily are already showing signs of chronic inflammation that make them at risk for HNSCC later on. With

more research, a saliva-based test could be a cost-effective tool in assisting early diagnosis of head and neck cancers through

promising associations between substance use and pro-inflammatory cytokines.

Biography

Miranda Li is an Honors High School Student who dedicates most of her free time to research.

Miranda Li et al., J Clin Exp Pathol 2017, 7:2 (Suppl)

DOI: 10.4172/2161-0681-C1-035