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Volume 6
Journal of Plant Biochemistry & Physiology
ISSN: 2329-9029
Plant Science 2018
October 29-30, 2018
October 29-30, 2018 | Valencia, Spain
6
th
Global Summit on Plant Science
Antagonistic endophytic
Streptomyces
strains, taxonomic characterization, plant growth promoting
activities, effect on cocoyam (
Xanthosoma sagittifolium
(L.) Schott) and biochemical constituents
Djuidje Kouomou P F
1, 2, 3
, Boudjeko T
1,2,3
, Asultan W
4
, Nouemssi S B
1
, Lerat S
3
, Beaulieu C
3
and
Wong Mui-Yun
4
1
Biotechnology Centre-Nkolbisson, Cameroon
2
University of Yaound , Cameroon
3
Universit de Sherbrooke, Canada
4
Universiti Putra Malaysia, Malaysia
I
n the present study, 7 endophytic actinobacteria were isolated from cocoyam’s roots. Due to their antagonistic activity against
P. myriotylum
and other plant pathogens, four isolates PERM1, PERM2, PERM4 and PERM5 were selected. These four
selected isolates were subsequently characterized taxonomically, evaluated for
in vitro
biocontrol traits, plant growth promoting
(PGP) activity and their effect was evaluated on cocoyam’s vitro plants. The antagonistic endophytic isolates were able to grow
in NaCl concentrations of up to 8%, at pH values between 5 and 9, temperatures between 25°C and 45°C. Of the four selected
isolates, PERM2 and PERM4 produced cellulase; PERM1 and PERM2 produced pectinase whereas all the selected isolates
produced chitinases, proteases, lipases and β- 1,3-glucanases. The selected isolates were able to solubilize phosphate, were
positive for siderophores, ACC deaminase, nitrogen fixation, ammonia (NH
3
) and indole-3-acetic acid (IAA) production. The
amount of IAA ranges between 12.53-20.30 μg/ml and amount of ammonia ranges between 261.71-633.42 mg/ml. Of the four
isolates, PERM1, PERM2 and PERM4 produced HCN. Based on sequences of 16S rRNA gene, the selected endophytic isolates
were identified as
Streptomyces
but different species in BLAST analysis. Regarding their effect on cocoyam’s root development,
in comparison to control only isolate PERM2 demonstrated the maximum increase in root length whereas PERM2; PERM4
showed a remarkable effect in number of primary and lateral roots of cocoyam’s vitro plants and
Streptomyces
sp. PERM2 and
PERM4 showed greatly increase in fresh cocoyam’s vitro plant weight. Biochemical assay revealed the highest amount of protein
content and peroxidase activity in roots inoculated with PERM1 and PERM5 compare to control. These results clearly suggest
the possibility of using endophytic
Streptomyces
strains from cocoyam’s roots as biocontrol agents against fungal phytopathogens
and as bio-inoculant for plant growth promotion for sustainable and environmentally friendly agriculture.
muiyun@upm.edu.myJ Plant Biochem Physiol 2018, Volume 6
DOI: 10.4172/2329-9029-C1-003