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Volume 6

Journal of Plant Biochemistry & Physiology

ISSN: 2329-9029

Plant Science 2018

October 29-30, 2018

October 29-30, 2018 | Valencia, Spain

Global Summit on Plant Science

J Plant Biochem Physiol 2018, Volume 6

DOI: 10.4172/2329-9029-C1-003

Identification of endophytic bacterial communities in Pakistani wheat

Tehmina Kiani, Nighat Noureen, Tayyaba Yasmine, Muhammad Zakriya

and

Sumaira Farrakh

COMSATS Institute of Information & Technology Islamabad, Pakistan

heat (

Triticum aestivum

) is among one of the major staple food, although cultivated world widely but very little is known

about the association of endophytic bacteria within the roots, stem and leaves. The main objective of this study was to

identify the culturable endophytic bacteria of wheat from roots, stem and leaves and analyze the diversity among different wheat

varieties (Punjab 2011, Galaxy 2013-2014, Inqilab 91, Faisalabad 2008, Ujala 2015, TW1312, TW1410, TW1415, 15BT023)

grown under the same field conditions. The density of endophytic populations varied from 6x10

to 1.4x10

CFU g

-1

of fresh

weight. Among the total 85 isolated endophytic bacteria, 37.2%were isolated from roots, 35.9% from stem and 26.9% from leaves.

Based on 16S rDNA sequences, 32 different strains were isolated comprising of 3 phyla, 5 classes and 12 sub classes. Among

these phyla, Firmicutes (44.7%) were most abundant, followed by Proteobacteria (30.6%) while the Actinobacteria (24.7%)

were isolated less frequently. The common genera among all the varieties and wheat lines include

Bacillus, Microbacterium,

Arthrobacterium, and Enterobacterium

. To access and compare the community structure, diversity indices were calculated. The

abundance and level of colonization by SEM (Scanning Electron Microscope) analysis also demonstrated that the endophytes

were more abundant in roots as compared to leaves. This study also indicates that the specific wheat varieties may also contribute

in the distribution of bacterial endophytes. Future studies will be required to determine the potential application of these isolates

in growth promotion, biological control, and enzyme production not only in wheat but also in other commercially important

crops to fulfill the food demand and to overcome the rate of poverty.