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Volume 7, Issue 6(Suppl) J Chromatogr Sep Tech
ISSN: 2157-7064 JCGST, an open access journal
Page 35
Notes:
Separation Techniques 2016
September 26-28, 2016
conferenceseries.com
Separation Techniques
September 26-28, 2016 Valencia, Spain
2nd International Conference and Expo on
A simple, accurate, time-saving and green method for the determination of 15 sulfonamides and
metabolites in serum samples by ultra-high performance supercritical fluid chromatography
Feng Zhang
Chinese Academy of Inspection & Quarantine, China
S ulfonamides are a large group of synthetic antibiotics which has anti-bacterial properties. They have a good antibacterial
effect on both Gram-positive and Gram-negative bacteria. Sulfonamides are commonly prescribed in human and veterinary
medicine against many kinds of infections. In order to investigate the pharmacodynamics and pharmacokinetics of different
sulfonamides, establishing a detection method of multiple sulfonamides and their metabolites in serum is necessary. An
analytical method based on ultra-high performance supercritical fluid chromatography (UHPSFC) with photo-diode array
detection (PDA) has been developed to quantify 15 sulfonamides and their N4-acetylation metabolites in serum. Under the
optimized gradient elution conditions, it took only 7 min to separate all 15 sulfonamides and the critical pairs of each parent
drug and metabolite were completely separated. Variables affecting the UHPSFC were optimized to get a better separation. The
performance of the developed method was evaluated. The UHPSFCmethod allowed the baseline separation and determination
of 15 sulfonamides and metabolites with a limit of detection ranging from 0.15 to 0.35 g/mL. Recoveries between 90.1 and
102.2% were obtained with satisfactory precision since relative standard deviations were always below 3%. Hence, the proposed
method is simple, accurate, time-saving and green; it is applicable to a variety of sulfonamides detection in serum samples.
Biography
Feng Zhang has completed his PhD from Dalian Institute of Chemical Physics, Chinese Academy of Sciences in the year 2005. He has done his Post-doctoral
research from Max Planck Institute of Biochemistry under the funding of Max-Planck Society. He has worked as a Senior Expert of Food Safety at the Chinese
Academy of Inspection and Quarantine. In 2014, he was assigned as the Director of the Institute of Food Safety and focusing on Chromatography/Mass
Spectrometry Techniques applied to food, tobacco, proteomics, metabolomics and pharmaceutical research. He has published more than 40 papers in reputed
journals, 4 books and has authorized 3 patents.
Feng Zhang, J Chromatogr Sep Tech 2016, 7:6(Suppl)