separation-techniques-2016_scientifictracks-abstracts

15 / 46

Volume 7, Issue 6(Suppl) J Chromatogr Sep Tech
ISSN: 2157-7064 JCGST, an open access journal
Page 39
Notes:
Separation Techniques 2016
September 26-28, 2016
conferenceseries.com
Separation Techniques
September 26-28, 2016 Valencia, Spain
2nd International Conference and Expo on
Development of a liquid-liquid extraction method of resveratrol from cell culture media using
solubility parameters
Mohamad Houssam Al Balkhi1, 2, Mohammad Amin Mohammad2, 3, Leo-Paul Tisserant1, 4 and Michele Boitel-Contia1
1Universite de Picardie Jules Verne, France
2University of Damascus, Syria
3University of Bradford, UK
4Universite de Reims Champagne-Ardenne, France
The extraction of bioactive compounds, produced by plant cell cultures, directly from their culture medium, which contains
other by-products, is a great challenge. Resveratrol extraction from its grapevine cell cultures is considered here as an
example to improve the extraction processes from plant cell cultures using solubility parameters. Successive liquid-liquid
extraction (LLE) processes were exploited to extract resveratrol from the culture medium with an extraction ratio approaching
100%, high selectivity and minimum amounts of solvents. The calculations of partition coefficients as a function of solubility
parameters demonstrated that benzyl benzoate is the most suitable intermediate solvent to extract resveratrol from its aqueous
medium at a benzyl benzoate/medium ratio of 1:100 v/v. The calculations also illustrated the high ability of methanol and
ethanol to extract resveratrol from benzyl benzoate. The physicochemical properties of benzyl benzoate and processing
conditions were exploited to separate it from aqueous media and organic solvents. The agitation method, component ratios and
extraction time were studied to maximize the extraction yield. Under the best studied conditions, the recovery of resveratrol
from different culture media approached approximately 100%with a selectivity of approximately 92%. Ultimately, the improved
extraction processes of resveratrol are markedly efficient, selective, rapid and economical.
Biography
Mohamad Houssam Al Balkhi has obtained his PhD in 2009 from the University of Picardie Jules Verne, France. He has worked as an Assistant Professor at the
University of Damascus, Syria for many years. Actually, he is a Post-doctoral Fellow at the University of Picardie Jules Verne working on Plant Biotechnology and
the development of extraction methods of active compounds. He has published many papers in reputed journals.
Mohamad Houssam Al Balkhi et al., J Chromatogr Sep Tech 2016, 7:6(Suppl)