traditional-medicine-2016_posters-accepted-abstracts

Volume 5, Issue 2 (Suppl)

J Tradi Med Clin Natur

ISSN: JTMCN, an open access journal

Page 92

Traditional Medicine 2016

September 14-16, 2016

conferenceseries

.com

September 14-16, 2016 Amsterdam, Netherlands

International Conference and Exhibition on

Traditional & Alternative Medicine

J Tradi Med Clin Natur 2016, 5:2 (Suppl)

Madhuram, a poly herbal formaulation in 3t3l1 cell lines augments glucose uptake and ameliorates

insulin resistance by regulating glucose transporter-4, peroxisome proliferator-activated receptor

- gamma gene expression

in vitro

Srivani Telapolu

Sri Ramachandra University, India

Back ground:

Major barrier for integrating CAM into modern clinical practice is the lack of the proof for quality control, safety and

efficacy through an evidence based approach. Identification of underlying mechanism of action of polyherbal formulations, have

generally not been elucidated due to the lack of knowledge in identifying their contained active phytochemical constituents. In our

present work we made an attempt to identify the possible mechanism of action of an antidiabetic poly herbal ‘Madhuram’ which is

standardized for its specific phytochemical markers.

Methods:

Methanol extract of the formualtion (MEF) was tested for cytotoxicity in 3T3L1 preadipocytes. 3T3-L1 preadipocytes,

were induced for adipocyte differentiation by adding differentiation media containing 0.5 mM/l of IBMX, 0.25 lM/l of DEX and 1

mg/l of insulin in DMEM medium with 10% FBS. Glucose uptake potential of MEF was estimated in differentiated adipocytes from

1-1000 µg/ml concentartion using Pioglitazone as a standard drug. For evaluating the adipogenic activity, MEF was added along

with differentiation media to preadipocyets at 32.5-500 µg/ml concentration and activity was measured by oil red O staining using

pioglitazone as negative control. For confirming the actual mechanism of MEF, regulation of PPAR gamma and Glut4 mRNA were

studied using RT-PCR analysis.

Results:

Cytoxicity of MEF is neglegable even at 1000µg/ml. At the tested doses, MEF increased glucose uptake in dose dependantly

and at 125 µg/ml it completely inhibited lipid droplet formation. PPAR gamma and glut4 expression is upregulated in a dose dependant

manner at 100, 200, 300 µg/ml concentration.

Conclusion:

Madhuram is showing antidiabetic activity by ameliorating insulin resistance through inhibiting the adipogenesis.