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Volume 6, Issue 4(Suppl)

Transl Med 2016

ISSN: 2161-1025, an open access journal

Page 70




Translation Medicine & World Oncologists 2016

November 28-30, 2016

Translational Medicine and Oncologists Meet

November 28-30, 2016 San Francisco, USA



Annual Conference on

In Adenocarcinomas (ACs) high Nitric Oxide (HNO) leads to adaptation of Tumor Stem Cell (TSC)

Juel Chowdhury


, Karla Licona, Humera Batooll, Saarah Alkhairy


James A Radosevich



University of Illinois, USA


Jesse Brown VAMC, USA


Adaptation of adenocarcinomas (ACs) after exposure to consistently high concentrations of Nitric Oxide (NO) after

adaptation evokes a transformation to an aggressively physical composition identical to cancer stem cells. This is owed to the varying

expression of specific biochemical pathways, in particular, cellular respiration being altered.


Cells modified to large concentrations of High Nitric Oxide (HNO) portray a more vigorous phenotype of AC homologous

to cancer stem cells in contrast to non-modified cells. The exact molecular and cellular mechanisms that occur during HNO exposure

are not fully understood. Further knowledge of these mechanisms can lead to the development of new drugs targeted against these

tumor stem cells.


The five human cell lines (Lung: A549; Breast: BT20, Hs578, MCF7, and T47D) were exposed gradually by increasing

quantities of HNO to the NO donor, DETA-NONOate. Parent and HNO cell lines were tagged with red/green fluorescent markers

and the isolated mRNA from both were used to compete in a human genome gene chip analysis experiment.


HK1 and COX7C were up-regulated genes whereas LDHB, LDHBP, PDHA1, AC004490.1, and LSM7 were continuously



On exposure to high concentrations of NO, the enzyme in the cellular respiration pathway that was most affected in

ADC cell lines was the PDHA1 (Pyruvate Dehydrogenase Alpha 1). This specific enzyme functions by breaking down Pyruvate into

Acetyl-CoA, affecting both Glycolysis as well as the Krebs Cycle. The decrease in the number PDHA1 enzymes implies that AC cells

have alternate pathways for energy (ATP) formation.


Juel Chowdhury began his professional education at the Gulf Medical University where he received his Bachelor of Medicine and Bachelor of Surgery degree. Since then,

his works have led him to partner and study with Nobel laureate Dr. Ferid Murad and many well-known scientists such as Dr. Robert Winn, Director of UI Health. He is the

Founder and President of

, an online professional network with an open access journal for the oncologists. His innovative iGenX Lab is a genetical research

lab based on data-mining and data analysis of the gene-chip experiment. He is an Editorial Board Member for many international journals like Tumor Biology, JCMT, JUMD,

and many professional societies like ASCO and ISOBM. He was the Director of ISOBM (International Society of Oncology and Biomarkers)-2016 Congress held in Chicago

and also the upcoming ISOBM 2017 Congress in Brazil. He is an expert in Botulinum Toxin and Dermal Fillers, Facial Reconstruction and Hair Transplant procedures. He

is a faculty member of National College of Health. His research interests are as follows: Head & Neck Cancer, Lung & Upper Aero Digestive Tumors, Human Tumor Stem

Cells, Nitric Oxide in Tumor Environment.

[email protected]

Juel Chowdhury et al., Transl Med 2016, 6:4(Suppl)