translational-medicine-and-oncologists-meet-2016_posters

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Volume 6, Issue 4(Suppl) Transl Med 2016
ISSN: 2161-1025, an open access journal
Page 69
Notes:
conferenceseries.com
Translation Medicine & World Oncologists 2016
November 28-30, 2016
Translational Medicine and Oncologists Meet
November 28-30, 2016 San Francisco, USA
14th Annual Conference on
Increased Nitric Oxide (NO) exposure in pseudogenes may play a role in cancer stem cell formation
Madhusudan Patel, Juel Chowdhury1, Mayela Leal, Mina Alsayyab and James A Radosevich1,2
1University of Illinois, USA
2Jesse Brown VAMC, USA
Hypothesis: The high exposure of CeRNA and microRNA to HNO effects the pseudogenes and the coding along causing their over
expression in cancer cells.
Objective: Evidence regarding pseudogenes being functional have recently emerged. MicroRNA isolation due to CeRNA adds to
pseudogenes and their influence on the growth of cancer. One well studied gene is the BRAF pseudogene and its functional BRAF gene.
Studies have shown that high levels of the BRAF pseudogene are directly proportional to the formation of aggressive malignancies.
Methods: Gene chip analysis of ten HNO adapted cell lines (Squamous cells: SCC-016, SCC-040, SCC-056, SCC-114, SCC-116;
Adenocarcinomas: A549, BT20, Hs578, MCF7, and T47D) was carried out. Known pseudogenes were identified in each line, as well
as their coding counterparts.
Results: The adenocarcinoma cell lines RP6-159A1.2, RP11-255N24.3, AC004490.1, LDHBP, RP11-572H4.2 were down regulated
pseudogenes, and there were no up regulated pseudogenes. The squamous cell carcinomas (SCCs) had the following up regulated
pseudogenes: RPL37AP1, AC138972.1, RP11-641D5.1, AC005534.6, AC022431.1, RPL26P12, and they had these down regulated
pseudogenes: RP6-159A1.2, RP11-255N24.3, RBMXP1, RP11-20O23.1, RP11-551G24.2. All cell lines followed the hypothesis, showing
an increase in a pseudogene expression indicating an increase in the corresponding gene (with the exception of the adenocarcinoma
cell lines).
Conclusions: The high concentration of CeRNAmay reduce expressions of microRNA, which would then lead to high concentrations
of pseudogenes (likely due to high levels of HNO). Pseudogenes, along with BRAF, in turn reduce the expression of microRNA.
Therefore, the pseudogenes and BRAF take the same role as the CeRNA. This results in a feedback loop of over expression of the
coding gene.
Biography
Madhusudan Patel is a Medical Doctor from Gujarat, India who began his medical education at University of Seychelles - American Institute of Medicine and graduated
in 2013. He travelled to UAE, Seychelles, Mauritius and India for clinical exposure, throughout his medical education. After moving to the United States, he has actively
pursued his medical education with many established doctors. He volunteered at the 43rd ISOBM Annual Conference held during September, 2016 in Chicago, IL, which
was attended by established academics such as Dr. Ferid Murad and Dr. Robert Winn. Recently, he has enthusiastically started developing his skills in medical research
and is a Board Member of where he is working towards helping other people who share similar fields of interest. He is a very passionate medical doctor who
aims to acquire a position in Internal Medicine residency program and is actively working towards stretching his academic boundaries.
Madhusudan Patel et al., Transl Med 2016, 6:4(Suppl)