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Volume 6, Issue 4(Suppl)

Transl Med 2016

ISSN: 2161-1025, an open access journal

Page 72




Translation Medicine & World Oncologists 2016

November 28-30, 2016

Translational Medicine and Oncologists Meet

November 28-30, 2016 San Francisco, USA



Annual Conference on

High nitric oxide levels in head and neck Squamous Cell Carcinomas (SCC) has shown down regulation

of Cadherin Type-1 (CDH1) gene

Saarah T Alkhairy, Ghassan Jibawi, Tahani Taj, Manpreet Arora, Mayela Leal, Ravikant Bhaskar


James A Radosevich



University of Illinois, USA


Jesse Brown VAMC, USA


In head and neck squamous cell carcinomas High Nitric O xide (HNO) levels cause down regulation of genes that

has metastatic potential.


Nitric oxide is a signaling molecule that in its chemical compound is a powerful vasodilator and a free-radical that all

cells in our body produce. At normal levels nitric oxide helps cells to communicate and transmit signals throughout the body. In

cancer patients’ high levels of nitric oxide are cytotoxic and low levels are considered cytostatic. Cell adhesion, cell signaling and

cell communication are regulated by protein coding genes and overexposure to HNO down regulates these genes. High nitric oxide

levels were presented in four of the H&N SCC cell lines, in order to study more on the relationship between HNO (high nitric oxide)

levels and its metastatic potential. It was observed that HNO in H&N SCC cell lines caused down regulation of CDH1 in all the four

cell lines. Abnormalities in tumor suppressor protein coding genes are one of the causes of carcinogenesis. In this case, CDH1 was

found to be down regulated. CDH1 gene provides a manual for producing a protein that code for epithelial cadherin or E-cadherin.

E-cadherin plays an important role as tumor suppressor gene as it regulates cell adhesion and cell proliferation


In this study we adjusted the H&N SCC cancer cell lines SCC016, SCC040, SCC056, and SCC114 to HNO. Nitric oxide

levels were increased to HNO by DETA-NONOate. DNA microarray was performed in order to compare the control cell line and

HNO cell line.


HNO levels in adapted H&N SCC cell lines caused CDH1 genes to be down regulated.


It was studied that adapting HNO to H&N SCC cell lines down regulates CDH1(Cadherin-1) gene which means that

the gene expression is either reduced or decreased which increases the metastatic potential. Further research is needed to observe the

relationship between carcinogenesis, CDH1 and levels of nitric oxide.


Saarah Alkhairy has received her Bachelor degree in Business Management from Purdue University, Indiana. Her goal is to combine healthcare and business to affect

society on a larger scale. This may include education, pharmaceuticals, and research. She published an abstract in the American Thoracic Society 2016 on PFT in Muscular

Dystrophy with Sleep Disordered Breathing in Children and Adolescents. The aim was to determine if the AHI correlates with abnormal PFTs in order to predict the need

for a nocturnal polysomnogram. She was an Associate Editor of Wikidoc and a Research Fellow at Perfuse Study Group in Beth Israel Deaconess Medical Center,

Massachusetts. She added content and edited highly read and searched topics including bronchiectasis, sleep apnea, interstitial lung disease, colorectal cancer, lung

cancer, and basal cell carcinoma for the world's largest medical wiki.

[email protected]

Saarah T Alkhairy et al., Transl Med 2016, 6:4(Suppl)