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Journal of Pharmaceutical care and Health Systems | ISSN: 2376-0419 | Volume 5
Pharmaceutical sciences
11
th
World Congress on
September 28-29, 2018 | Montreal, Canada
Is Quercetin genotoxic or anti-genotoxic in the presence of divalent metals?
Fatemeh Soltani Sarah Beiki, Hasan Zarei
and
Mehrdad Iranshahi
Mashhad University of Medical Sciences, Iran
S
ome of the natural antioxidants such as quercetin exhibit pro-oxidant effects in the presence of divalent metal ions including
Iron (Fe (II)), Copper (Cu (II)), Zinc (Zn (II)), and etc. On the other hand, these metals can induce oxidative DNA damage
that subsequently results in several health disorders. In recent years, consumption of natural based antioxidants have been
increased, therefore the existence of metal ions in the body (either from endogenous or exogenous sources) may neutralize the
antioxidant effects of these compounds and even in some circumstances it might convert the antioxidant into the pro-oxidant
effects and subsequently cellular damage. Accordingly, it is necessary to determine the behavior of these substances, namely
protection or damage in the presence of divalent metals. The aim of this study was to evaluate the probable genotoxicity or
anti-genotoxicity effects of Quercetin in the presence of metal ions using come assay in the NIH 3T3 cell line. The genotoxic
activity of quercetin (2.5, 5, 10, 25, 50, 100 and 200 µM) alone and in combination with divalent metal ions (50 µM) in NIH 3T3
cells was evaluated by Comet assay. The treated cells were incubated at 37°C for 1hour. The damage severity was measured by
scoring the cells following calculating of % DNA in Tail using comet score software. The results showed that quercetin at tested
concentrations showed negligible DNA damage (1.3-2 % DNA in tail), however divalent metals including Iron, Copper, and
Zinc (50 µM) exhibited significant genotoxicity (31-33 % DNA in tail). According to the results, Quercetin at concentrations
of 10 to 100 µM (**p0.01) could protect the cells against DNA damage induced by metals. This study showed that Quercetin at
some concentrations has the protective effect against oxidative DNA damage, however further studies,
in vitro
and
in vivo
are
needed to evaluate the exact properties and behaviors of this compound.
Biography
Fatemeh Soltani got her PhD in pharmaceutical biotechnology from Mashhad University of Medical Sciences, Iran in 2013. As her PhD thesis, she worked on
some recombinant fusion peptides as gene delivery vectors. During her PhD, she was involved in several projects other than her thesis which allowed her to
gain experience in various practical techniques such as genotoxicity, cytotoxicity, gene cloning, protein expression, protein purification, fusion protein design,
nanoparticle formation, characterization and etc. In addition, she spent a seven-month sabbatical in 2010 at the University of Queensland in Australia. During that
period, she worked on the synthesis of lipid-peptide core nanoparticles by using solid phase peptide synthesis (SPPS) methods. At the moment she is an assistant
professor in the Department of Biotechnology, School of Pharmacy, Mashhad University of Medical Sciences, Iran.
Fatemeh Soltani Sarah Beiki et al., J Pharma Care Health Sys 2018, Volume 5
DOI: 10.4172/2376-0419-C3-033