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Journal of Pharmaceutical care and Health Systems | ISSN: 2376-0419 | Volume 5
Pharmaceutical sciences
11
th
World Congress on
September 28-29, 2018 | Montreal, Canada
In-vitro Antileishmanial activity of
Artemisia dracunculus
and
Heracleum persicum
extracts in
comparison with Glucantime
Batool Sadeghi-Nejad
Abadan School of Medical Sciences, Iran
Background & Objectives:
Cutaneous leishmaniasis (CL) is one of the most common parasitic diseases. It is one of the major
public health in developing countries and throughout the world. Pentava¬lent antimonial compounds like pentostam and
Glucantime has been used to treat CL for the last 50 years. The use of these compounds has some limitations such as long
duration of treatment, high expenses of drugs, and methods of drug use which are intradermal and intramuscular injection.
Beside these, lack of response to the treatment in 10-15% of cases and toxic effects on heart, liver, and kidneys are other possible
side effects [1-3]. Hence, the objective of the present survey was to state the antileishmanial activity of two herbal medicine
(
Artemisia dracunculus
and
Heracleum persicum
) extracts were evaluated against
Leishmania major and Leishmania infantum
using colorimetric MTT (2-(4,5-dimethyl-2-thiazolyl)-3,5-diphenyl-2H-tetrazolium bromide) assay and compared to the
Glucantime as a reference. Materials and Methods: The leaves extracts of selected plants were obtained by maceration. The
in
vitro
assays were carried out on
L. major
and
L. infantum
using colorimetric MTT assay in comparison with Glucantime. The
concentration-response curves tested extracts and Glucantime solutions were designed and IC50 values were located.
Results:
Anti-Leishmina effects of
A. dracunculus
and
H. persicum
) on
L. major
and
L. infantum
promastigote were revealed
with 50% inhibitory concentration (IC50) values of 49.67 and 42.23 mg ml-1 for
A. dracunculus,
81.15 and 73.17 mg mg ml-1
for H.
persicum
. In the comparison with the standard drug, glucantime, which had IC50 value of 40.2 mg ml-1 for L.major and
18.5 mg ml
-1
for
L. infantum
promastigote after 72 hours incubation, respectively.
Conclusion:
These results revealed that compounds from Satureja khuzestanica and
Heracleum persicum
have anti-leishmania
properties that necessary to survey the effects of these extracts on leishmania genus in animal models in future.
Biography
Batool Sadeghi-Nejad Graduated of bachelor degree (B. Sc.) in the field of public health from Isfahan University of Medical Sciences on Feb. 15 1988. And he is
a holder of master degree (M. Sc.) in the field of Medical Parasitology from Isfahan University of Medical Sciences on April 30 1997 and he completed his Ph.D.
degree in Medical Mycology from Botany department of Pune University, India on Feb. 2008. She is members of Scientist Academic Institution, Abadan School of
Medical Sciences, Abadan, Iran (Assistant professor, Ph.D Medical Mycology). She has published more than 24 papers in reputed journals and has been serving
as a reviewer board member of repute.
Batool Sadeghi-Nejad, J Pharma Care Health Sys 2018, Volume 5
DOI: 10.4172/2376-0419-C3-033