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Volume 9 Chemical Sciences Journal Asia Chemistry 2018
September 12-13, 2018
11th Annual Congress on
September 12-13, 2018 Singapore Chemistry
Analysis of Human Milk Oligosaccharides from Filipino breastmilk reveals secretor status variation
Gladys Cherisse J. Completo1, Anthony Salvador B. Albaladejo2, Ruel C. Nacario1, Baby Richard R. Navarro3, Carlito B. Lebrilla4
1Institute of Chemistry, University of the Philippines Los Baños, Laguna 4031
2Department of Chemistry, University of the Philippines Visayas, Miagao, Iloilo 5023
3Institute of Food Science and Technology, University of the Philippines Los Baños, Laguna 4031
4Department of Chemistry, and Biochemistry (School of Medicine), University of California, Davis, CA 95616
Human Milk Oligosaccharides (HMOs), although lacking in nutritional value and considered indigestible to the infant,
serve many infant-health-beneficial functions, such as nourishment of gut bacteria, inhibition of pathogenic infection,
and aiding in brain development. Their expression depends on a series of competing Glycosyltransferases driven by the
secretor gene and Lewis blood group. In this study, aiming to determine possible correlation between HMOs and secretor
status, a rapid- throughput 96-wellplate method, entailing lipid separation by centrifugation, protein precipitation using
Ethanol, Alditol sugar reduction using Sodium Borohydride, and solid phase extraction purification using porous graphitized
carbon, was used to analyze HMOs from Filipino breastmilk. A Nano-HPLC Chip/TOF- MS coupled with the use of Agilent
MassHunter programs and an in-house library was used to analyze purified HMOs. Five HMO secretor markers were also
used to quantify the level of α(12) fucosylation. From the results, 81% of the mothers sampled were determined to be secretors
and 19% to be non- secretors; ,Moreover, the secretor mothers were found to produce milk richer in Oligosaccharides than the
non-secretormothers. However, variations in Oligosaccharide type, which could be linked to variations in secretor status, were
observed in both. Thus, this study provided a rapid method for determining phenotypic secretor status using HMO markers
from Filipino breastmilk samples.
Biography
Gladys C.J. Completo is a Professor at the University of the Philippines Los Baños. Her field of interests includes Carbohydrate synthesis, Glycomics and
Glucoproteomics of milk Oligosaccharides.
Gladys Cherisse J. Completo et al., Chem Sci J 2018, Volume 9
DOI: 10.4172/2150-3494-C5-030