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Volume 5, Issue 3(Suppl) Biochem Anal Biochem 2016
ISSN: 2161-1009, Biochem an open access journal
Page 30
Notes:
Biochemistry 2016
October 10-12, 2016
conferenceseries.com
Biochemistry
October 10-12, 2016 Kuala Lumpur, Malaysia
International Conference on
Thymoquinone attenuates cisplatin induced toxicity and oxidative damage in rat kidney
Zeba Farooqui, Faaiza Shahid and Farah Khan
Aligarh Muslim University, India
Nephrotoxicity is a severe complication in patients undergoing cisplatin (CP) chemotherapy. Thymoquinone (TQ), a monoterpene
isolated fromvolatileoil of Nigella sativa seeds has beenshown toexhibit strongantioxidant properties andprotective effects against
oxidative damage induced by several drugs and toxicants. The present study was undertaken to investigate whether TQ can prevent the
CP-induced nephrotoxic effects or not. Rats were divided into four groups: Control, CP, TQ and CP+TQ. Rats in the groups CP+TQ
and TQ were administered TQ (1.5 mg/kg bwt orally), prior to and simultaneously with and without, multiple doses of CP (3 mg/kg
bwt, i.p) every fourth day for 20 days, respectively. CP nephrotoxicity was evident by increased serum creatinine (Scr) and blood urea
nitrogen (BUN). CP treatment caused oxidant/antioxidant imbalances as reflected by increased lipid peroxidation (LPO), decreased
enzymatic and non-enzymatic antioxidants. Furthermore, the activities of brush border membrane (BBM) marker enzymes like
alkaline phosphatase (ALP), γ-glutamyl transferase (GGTase) and leucine aminopeptidase (LAP) were significantly declined in renal
cortical and medullary homogenates and in isolated BBM vesicles (BBMV) in CP treated rats. Oral TQ administration, significantly
attenuated CP induced increase in Scr and BUN and decrease in BBM enzymes activities. TQ administration also precluded CP
induced alterations in the enzymatic and non-enzymatic antioxidant parameters. Histopathological observations showed extensive
kidney damage in CP treated animals and remarkably reduced renal injury in CP and TQ co-treated group. The results suggested
that TQ alleviates CP induced nephrotoxicity and oxidative damage by strengthening antioxidant defense mechanism in the kidney.
Biography
Zeba Farooqui is currently pursuing PhD at the Department of Biochemistry, Aligarh Muslim University (AMU), India. She is currently working on “protective effect of Nigella
sativa and thymoquinone on cisplatin induced toxicity in rat kidney”. She has published a research article in an international journal. She has presented her work in several
scientific meetings and conferences.
Zeba Farooqui et al., Biochem Anal Biochem 2016, 5:3(Suppl)