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Volume 5, Issue 3(Suppl) Biochem Anal Biochem 2016
ISSN: 2161-1009, Biochem an open access journal
Page 27
Notes:
Biochemistry 2016
October 10-12, 2016
conferenceseries.com
Biochemistry
October 10-12, 2016 Kuala Lumpur, Malaysia
International Conference on
The correlation between malondialdehyde concentration level in seminal fluid analysis and
asthenozoospermia among infertile males
Debora Roselita Karo Sekali
University of Indonesia, Indonesia
In Indonesia, approximately 20% of couples are infertile and 40% of the cases caused by male infertility. Impaired sperm motility
(asthenozoospermia) is the leading cause of inability to conceive after regular unprotected sex in one year. Reactive Oxygen Species
(ROS) is associated with male reproductive health. However, biochemical analysis on infertile males are rarely done in a clinical
practice andWHO laboratory manual ranges of seminal fluid characteristic can be different in various races and ethnicities. Objective
of this study is to compare malondialdehyde concentration as an indicator of ROS level in seminal plasma between normozoospermia
and asthenozoospermia and to find the correlation between level of malondialdehyde and sperm motility among infertile males.
Case control study analyzes 15 asthenozoospermic males and 20 normozoospermic males’ seminal plasma in a fertility clinic in
Jakarta. Thiobarbituric acid assay is used to measure the concentration of malondialdehyde. Mann-Whitney test shows, there is no
correlation between concentration of malondialdehyde and asthenozoospermia p value=0.194. However, average of malondialdehyde
concentration in normozoospermia is lower than in asthenozoospermia. The future research with the same topic should be done by
choosing fertile men as the control group and by taking minimal two samples for each subject since the population variances are
unequal.
Biography
Debora Roselita Karo Sekali has completed her Bachelor in Medical Science from Universitas Indonesia in 2016. She is currently pursuing her Honors Degree in Research
of Reproductive Physiology of Women with prolonged labor in Monash University, Australia.
Debora Roselita Karo Sekali, Biochem Anal Biochem 2016, 5:3(Suppl)