biochemistry-2016_scientifictracks-abstracts

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Volume 5, Issue 3(Suppl) Biochem Anal Biochem 2016
ISSN: 2161-1009, Biochem an open access journal
Page 28
Notes:
Biochemistry 2016
October 10-12, 2016
conferenceseries.com
Biochemistry
October 10-12, 2016 Kuala Lumpur, Malaysia
International Conference on
Pyruvate kinase as a new target for anti-caries agents
Palina Vyhouskaya, Wirginia Krzyściak, Anna Jurczak, Dorota Koscielniak and Ryszard Drozdz
Jagiellonian University, Poland
Background: In oral cavity conditions, cariogenic bacteria Streptococcus mutans are characterized by altered metabolism compared
to cells found in physiological flora. The metabolism of Streptococcus mutans is based on glycolysis, which also occurs in presence
of oxygen (a phenomenon known as the Warburg effect). The low concentration of oxygen (<2%), i.e., hypoxia inside the biofilm,
increases expression of genes encoding glycolytic enzymes and inhibits the oxidative phosphorylation. Pyruvate kinase (PK), one
of the enzymes involved in glycolysis, is considered as an enzyme conditioning the rate of the whole process since it is activated by
glucose-6-phosphate, a substrate of glycolysis.
Material & Methods: Pyruvate kinase from S. mutans ATCC and 40 clinical strains was purified, precipitated and estimated
fluorimetrically. Here, we revealed the activity and regulation of PK in mixed bacterial biofilm species, and discussed how these
properties enabled the regulation of PK for cariogenic biofilm proliferation and caries progression consequently. Clinical strains were
isolated from children with caries. Mixed biofilm assay was carried out according to current protocols in microbiology.
Results: PK activity was higher (1.65 mU/mg of protein) in the mixed cariogenic biofilm species compared to the single and mixed
physiological biofilm types (1.15 mU/mg of protein vs. 1.33 mU/mg of protein).
Conclusions: It was demonstrated that the pyruvate kinase activity is increased in mixed cariogenic biofilm species. Streptococcus
mutans are more resistant to glycolytic enzyme inactivation occurring in mixed cariogenic biofilm species (including Streptococcus
sobrinus, Lactobacillus acidophilus and Actinomyces viscosus ) compared to mixed physiological biofilm types. Inhibition of glycolytic
enzymes might be an essential step in the reduction of mixed cariogenic biofilm species which could be a useful tool in caries
prophylaxis.
Biography
Palina Vyhouskaya is currently a PhD student at the Jagiellonian University, Poland. She is a Member of the Scientific Students Association of Laboratory Diagnosticians,
where she gains experience and practice connected with modern research methods used in Medicine.
Palina Vyhouskaya et al., Biochem Anal Biochem 2016, 5:3(Suppl)