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Dementia 2016
September 29-October 01, 2016
Volume 6 Issue 5(Suppl) J Alzheimers Dis Parkinsonism 2016
ISSN:2161-0460 JADP, an open access journal
conferenceseries .com
September 29-October 01, 2016 London, UK
5th International Conference on
Alzheimer’s Disease & Dementia
Zhongxiao Wan et al., J Alzheimers Dis Parkinsonism 2016, 6:5(Suppl)
The combination of 1α,25dihydroxyvitaminD3 with resveratrol prevents endoplasmic reticulum
stress-mediated neuronal degeneration by improving insulin signaling and inhibiting tau
hyperphosphorylation in SH-SY5Y cells
Zhongxiao Wan, Jinbo Cheng and Liqiang Qin
Soochow University, China
Endoplasmic reticulum stress (ER stress) is a critical factor involved in the pathogenesis of AD. Vitamin D and resveratrol
are two nutritional factors that have reported neuroprotective effects, and findings from cellular model suggest that
resveratrol could potentiate vitamin D’s effects. We aimed to determine the effects of vitamin D & resveratrol on ER stress
mediated neurodegeneration and whether synergistic effects existed. Tunicamycin (2μM) was utilized to induce ER stress in
SH-SY5Y cells, cells were then incubated with vitamin D (10-7, 10 -8 and 10 -9 M) and resveratrol (25μM). The combination of
vitamin D & resveratrol completely reversed tunicamycin induced cytotoxicity in SH-SY5Y cells, as well as elevation in ER
stress markers (i.e.GRP78, p-eIF2α and CHOP), insulin signaling disruption (i.e. elevation in p-IRS-1serine307 and reduction
in p-Akt serine473) and tau phosphorylation (i.e. reduction in p-GSK3βserine9, and elevation in p-Tau serine396 &404).
Further studies are required to clarify whether the observed synergistic effects in the present study would also existed in vivo,
this will lay scientific foundation whether the combination of vitamin D with resveratrol might be an effective maneuver in the
prevention and treatment of AD in human subjects.
Biography
Zhongxiao Wan has completed her PhD at the age of 29 years from University of Alberta and postdoctoral studies from University of British Columbia. She is now
working at Soochow University as a research fellow. She has published approximately 16 papers in reputed journals in obesity and Alzheimer's disease related
fields.