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Journal of Clinical & Experimental Pharmacology | ISSN: 2161-1459 | Volume: 8
International Conference on
July 18-19, 2018 | Atlanta, USA
Pharmacology and Ethnopharmacology
11
th
International Conference and Exhibition on
Pharmaceutical Oncology
&
Isolation and identification of antifungal compounds from turmeric (Ryudai gold) and their activities
against
Fusarium solani
Jesmin Akter, Md Amzad Hossain
and
Sano Ayako
University of the Ryukyus, Japan
R
ecently, synthetic fungicide is gradually restricted due to its undesirable impacts on the environment and human health. Turmeric
(
Curcuma
spp.) has numerous biological activities including anticancer, antibacterial, antifungal and insecticidal properties.
There are more than 80 species of turmeric and 70 strains/varieties of
Curcuma
longa
with different chemical properties which
may possess different activities.
Fusarium solani sensu lato
(FSSL), pathogenic fungal species, causes several diseases in human,
animals, and plants. To date, there has been no report that addresses the effect of turmeric on F. solani. Therefore, we evaluated
antifungal activities of 3
Curcuma
longa strains (Ryudai gold: RD, Okinawa ukon, and BK2),
C. xanthorrhiza, C. aromatica, C.
amada and C. zedoaria
on 4 isolates of FSSL derived from American manatees (Trichechus manatus) with 3 different genotypes.
The methanol extract of all turmeric inhibited fungal growth concentration-dependently. Among different species and varieties
of turmeric,
Curcuma
longa
(Ryudai gold) had a highest inhibitory effect on fungal growth. For this, Ryudai gold was chosen for
isolation of antifungal compounds using silica gel column and high-performance liquid chromatography. Structural identification
of the antifungal compounds was conducted using
1
H NMR,
13
C NMR, and liquid chromatography-tandem mass spectrometry. The
purified antifungal compounds were curcumin (1), demethoxycurcumin (2), bisdemethoxycurcumin (3) and (
E
)-α-atlantone (4).
The order of the IC
50
against
F. solani
was curcumin (65-76 µM) > demethoxycurcumin (76-88 µM) > (E)-α-atlantone (91-118 µM)
>bisdemethoxycurcumin (711-746 µM). The results suggested that turmeric strain Ryudai gold developed by the University of the
Ryukyus showed excellent antifungal activities against FSSL and could be used for an antifungal agent.
jesminbau02@gmail.comClin Exp Pharmacol 2018, Volume: 8
DOI: 10.4172/2161-1459-C1-029