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Page 58
Journal of Clinical & Experimental Pharmacology | ISSN: 2161-1459 | Volume: 8
International Conference on
July 18-19, 2018 | Atlanta, USA
Pharmacology and Ethnopharmacology
11
th
International Conference and Exhibition on
Pharmaceutical Oncology
&
Ameliorative effect of ethanolic extract ofAnnonamuricata against sodiumarsenite: Induced hepatotoxicty
in Wistar rats
Adegboyega Adenike M
1,2
, OA Odunola
1
, KA Akinwumi, O Babalola
2
and
Akinwande B
1
1
University of Ibadan, Nigeria
2
The Polytechnic, Ibadan, Nigeria
I
ngestion of arsenic in drinking water causes cancer in multiple tissues and there is no cure. Cancer has become a great monster
to the human race, as it places a significant emotional and economic burden on families and governments all over the world. For
the management of arsenicosis, research is directed at chemoprevention using medicinally. In this study, we evaluated the
in-vitro
antioxidant and protection offered by Annona muricata L. (AM) against sodium arsenite-induced hepatotoxicity in rats. Antioxidant
and radical scavenging activities of AM were compared to vitamin C and Butylated Hydroxytoluene (BHT). Proximate and
phytochemical analyses were also carried out. Hepatoprotective study was investigated with six groups of rats that received distilled
water (Control), 5.0 mg/kg bwt (body weight) of NaAsO
2
, 250 mg/kg bwt AM, 500 mg/kg bwt AM, NaAsO
2
plus 250 mg/kg AM,
NaAsO
2
and 500 mg/kg AM. The NaAsO
2
was given once on days 7, 14 and 21, while AM was administered orally daily for 21 days.
Serum transaminases and alkaline phosphatase activities were determined and liver histopathology carried out. AM contained 2.00%
ash, 1.94% crude fat, 25.65% crude fibre, 2.88% protein and 58.62% carbohydrate. Phytochemical analysis indicated the presence of
alkaloids, flavonoids, and cardiac glycosides. The reducing power and metal chelating ability were in the order Vitamin C > BHT >
AM, while for DPPH scavenging ability AM > Vitamin C > BHT. The NaAsO
2
significantly (p < 0.05) increased the liver function
enzymes relative to control. However, reduction of the marker enzymes and restoration of the severe vacuolation of hepatocytes in the
NaAsO
2
group to near normal is being markedly done with the help of AM treatment. Our findings suggest that AM may constitute
a remedy against arsenic-induced hepatic injuries.
tijanimadinatu@yahoo.comClin Exp Pharmacol 2018, Volume: 8
DOI: 10.4172/2161-1459-C1-029