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Journal of Obesity & Weight Loss Therapy | Volume: 08
August 28-29, 2018 | Paris, France
Obesity, Diet and Nutrition
17
th
International Conference on
FMT reduces the mortality of BALB/c mice caused by
Listeria monocytogenes
(EGD-e) infection
Qing Liu
and
Liang Guo
University of Shanghai for Science and Technology, China
L
isteria monocytogenes
(Lm)
is a kind of food pathogenic bacteria with strong pathogenicity that has been shown previously
to cause infection via the gastrointestinal (GI) tract. External pathogens can cause changes in gut microbiota, and such a
change can promote or confer resistance to the infection of pathogenic bacteria. However, the changes in the microbiota during
Lm through the GI tract and infect the body is unknown. Eight-week-old mice’s were inoculated orally with
L. monocytogenes
EGD-e, and portions of the liver, spleen and cecal contents were removed, homogenized and plated, and feces were collected on
0 day, first day and third day. After that, different concentrations of FMT were used to treat Lm infected mice.
L. monocytogenes
culture confirmed that the content of Lm in cecum after intragastric inoculation reached the highest level on first day, and then
remained at a low level. The content of Lm in spleen and liver reached the highest level on third day. The percentage of the
Proteobacteria
spp,
Bacteroidetes
and
Cyanobacteria
on first day remained significantly higher than that of the 0 day (P<0.01),
while the proportion of
Lactobacillus
and
Staphylococcus
on first day was significantly lower than that of the 0 day (P<0.01).
Compared to first day, the
Coprococcus
,
Blautia
and
Eubacterium
increased significantly on third day. In addition, the mortality
of infected mice was reduced by 28% after FMT treatment compared with PBS treatment. Finally, we showed that inoculated
with EGD-e significantly altered the gut microbiome in mice in different times and the potential probiotics increased in
infected mice like the
Blautia
may be developed as new probiotics to enhance resistance against
L. monocytogenes
infections.
The gut microbiome of healthy mice can significantly reduce the mortality of infected mice by reducing the inflammatory
response and rebuilding the dysbacteriosis.
Biography
Qing Liu is a Director of institute for foodbone pathogen harmfuless and management school of medical instrument and food engnieering and Professor at
Univercity of Shanghai for Science and Technology, China
liuq@usst.edu.cnQing Liu et al., J Obes Weight Loss Ther 2018, Volume: 08
DOI: 10.4172/2165-7904-C5-070