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Journal of Obesity & Weight Loss Therapy | Volume: 08

August 28-29, 2018 | Paris, France

Obesity, Diet and Nutrition

International Conference on

Endocrine activities of adipocytes extracted from subcutaneous and visceral adipose tissues of obese

animals induced by high fat diet and supplemented with omega 3 fatty acid

Roberta Dourado Cavalcante da Cunha de Sá, Cruz M M, Batini F, Barbosa N, Farias T S M and Alonso Vale M I C

Federal University of São Paulo, Brazil

he efforts dedicated to finding the cure for obesity and associated disorders lead to an intense interest in adipocyte

metabolism. The consumption of ω-3 fatty acids (FA) presents beneficial effects on changes caused by obesity. The aim

of this study was to investigate the adipokines secretion of isolated adipocytes from obese mice induced by high fat (HF)

diet, supplemented or not with fish oil (FO) [rich in ω-3 FA (EPA/DHA, 5:1)] with emphasis on the differential response of

subcutaneous and visceral adipose deposits, inguinal (ING) and retroperitoneal (RP) region, respectively. C57BL/6J mice

received control (CO) or HF diet for 8 weeks. Supplementation with FO (2 g/Kg p.c., 3 times/week) was initiated 8 weeks after

the induction of obesity, remaining until the end, totaling 16 weeks of experimental protocol. The white adipose tissue ING

and RP were removed for isolation of adipocytes that were subjected to D'MEM / 10% FBS culture for 30 hours. At the end,

adipokines concentrations in the culture supernatant were determined using specific ELISA kits. The adipocytes of the HF

group showed a significant hypertrophy followed by an increase in the secretion of proinflammatory cytokines TNF-α and IL-6

compared to the CO group, whereas the HF + FO group presented total reversion of this effect, in both ING and RP adipocytes.

There was no difference in secretion of adiponectin. The relevance of isolated adipocytes in the secretion of these cytokines is

highlighted here. The adipocytes are affected by the HF diet and the FO has a protective effect on these parameters.

Biography

Roberta Dourado Cavalcante da Cunha de Sá has completed her PhD from Federal University of São Paulo. She is a Pharmacist, Master of Science and has

published one paper as first author and three papers as co-author.

Roberta Dourado Cavalcante da Cunha de Sá et al., J Obes Weight Loss Ther 2018, Volume: 08

DOI: 10.4172/2165-7904-C5-070