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Volume 6
Journal of Plant Biochemistry & Physiology
ISSN: 2329-9029
Plant Science 2018
October 29-30, 2018
October 29-30, 2018 | Valencia, Spain
6
th
Global Summit on Plant Science
A novel
gossypium barbadense
ERF transcription factor, GbERFb, regulation host response and
resistance to
verticillium dahliae
in tobaaco
Hanshuang Zhang, Jianguang Liu, Yongqiang Wang, B.S, Institute of Cotton Research, Hebei,China Guiyuan Zhao, Junli Zhao, Haiying Du
and
Xiaoliang He
Institute of Cotton Research, China
E
thylene-responsive factors (ERFs) are commonly considered to play important role in pathogen defense responses.
However, only few of ERFmembers have been characterized in Sea island cotton (
Gossypium barbadense
). Here we reported
a novel AP2/ERF transcription factors gene, named GbERFb which was cloned and identified from
Gossypium barbadense
by RACE. The expression of GbERFb was significantly up-regulated by ET, MeJA, SA, wounding, H
2
O
2
and
Verticillium
dahliae. Bioinformatics analysis showed that GbERFb protein contain a conserved ERF DNA binding domain and a nuclear
localization signal sequence. Further exprements demonstrated that GbERFb could be bind to GCC box cis-acting element and
interact with the GbMAPKb (MAP kinase) directly in yeast. Over-expression of GbERFb in tobacco could increase the disease
resistance to
V.dahliae
. These results suggest that the GbERFb, encode an AP2/ERF transcription factor, could be utilized for
enhancing
V.dahliae
tolerance.
Hanshuang Zhang et al., J Plant Biochem Physiol 2018, Volume 6
DOI: 10.4172/2329-9029-C1-003
Figure 1:
The full lenth cDNA sequence and deduced
amino acid sequence of GbERFb. The ERF domain
was single underlined, deduced nuclear localization
signal was shown in the grey box, The predict
phosphorylated sites were shown in the white boxes.
Figure 2:
The conserved ERF domain of GbERFb with those of
other closely related ERF proteins including AdERF13 (ADJ67442),
AtERF5(NP_568679), DcERF1 (BAF75651), GmERF(AAQ10777),
GmERF7(AEQ55266), JERF1(AAK95687),MtERF(XP_013467284),
NtERF4(NP_001312428),slERF5(NP_001234512), cERF2(EOY22776).
The three β-sheets and one α-helix of the ERF domain are indicated over
the corresponding sequences.