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Volume 6
Journal of Plant Biochemistry & Physiology
ISSN: 2329-9029
Plant Science 2018
October 29-30, 2018
October 29-30, 2018 | Valencia, Spain
6
th
Global Summit on Plant Science
Detection and verification of SNP related to cotton fiber quality in cotton cellulose synthase gene
family
Xiangyun Zhang
1
, Jinfa Zhang
2
, Jianhong Zhang
1
, Sujun Zhang
1
, Shuxin Shi
1
, Jie Chen
3
, Zongfu Han
4
, Jiwen Yu
5
, Jianyong Wu
5
, Chaozhu Xing
5
and
Xihua Li
5
1
Institute of Cotton Research, China
2
New Mexico State University, USA
3
Huazhong Agricultural University, China
4
Cotton Research Center, China
5
ICR - CAAS, China
C
ellulose synthases (CesAs) are multi-subunit enzymes associated with the plasma membrane in plants, playing a pivotal
role in cellulose production. In this study, the CesA gene coding sequences of four cotton species with sequenced genomes
were aligned. The phylogenetic analysis indicated that the CesA gene family in
G. arboreum, G. raimondii, G. hirsutum
(“TM-
1”) and
G. barbadense
(“Xinhai 21”) or
G. barbadense
(“3-79”) could be divided into 6 groups and 15 sub-groups, each group
containing 2-5 homologous genes. A total of 544 SNPs were identified in the CesA gene family among the five cotton genomes,
including 155 with including amino acid changes. An expression analysis of CesA genes through RNA-seq showed that one to
four GhCesA genes were Differentially Expressed (DE) in 0 and 3 DPA ovules between the two BILs (NMGA-062 and NMGA-
105) with different fiber lengths, but no DE gene was identified in 10 DPA fibers. Some SSR markers related to fiber quality
were found around CesA Genes. Part of SNPs in the differentially expressed GhCesA genes was checked for fiber quality (in
progress).
Xiangyun Zhang et al., J Plant Biochem Physiol 2018, Volume 6
DOI: 10.4172/2329-9029-C1-003