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Volume 6 Journal of Plant Biochemistry & Physiology
ISSN: 2329-9029 Plant Science 2018
October 29-30, 2018
October 29-30, 2018 | Valencia, Spain
6th Global Summit on Plant Science
Recent Publications
1. Meng, X., Li, F., Liu, C., Zhang, C., Wu, Z. and Chen, Y. (2010) Isolation and Characterization of an ERF Transcription
Factor Gene fromCotton (Gossypium barbadense L.). Plant Mol Biol Rep, 28, 176-183. doi:10.1007/s11105-009-0136-x
2. Guo, W., Jin, L., Miao, Y., He, X., Hu, Q., Guo, K., Zhu, L. and Zhang, X. (2016) An ethylene response-related factor,
GbERF1-like, from Gossypium barbadense improves resistance to Verticillium dahliae via activating lignin synthesis.
Plant Mol Biol, 91, 305-318. doi:10.1007/s11103-016-0467-6
3. Li, C., He, X., Luo, X., Xu, L., Liu, L., Min, L., Jin, L., Zhu, L. and Zhang, X. (2014) Cotton WRKY1 Mediates the Plant
Defense-to-Development Transition during Infection of Cotton by Verticillium dahliae by Activating JASMONATE
ZIM-DOMAIN1 Expression. Plant Physiol, 166, 2179-2194. doi:10.1104/pp.114.246694
4. Cai, Y., Xiaohong, H., Mo, J., Sun, Q., Yang, J. and Liu, J. (2012) Molecular research and genetic engineering of resistance
to Verticillium wilt in cotton: A review. African Journal of Biotechnology, 8, 7363-7372.
5. Zhang, Z., Zhao, J., Ding, L., Zou, L., Li, Y., Chen, G. and Zhang, T. (2016) Constitutive expression of a novel
antimicrobial protein, Hcm1, confers resistance to both Verticillium and Fusarium wilts in cotton. Sci Rep-Uk, 6,
20773. doi:10.1038/srep20773.
Biography
Hanshuang Zhang, professor, female, born in Hebei province. China. She has engaged in cotton breeding for over 20 years, and won the Technological Progress
First Prize Award of Hebei province. Her team has successively presided and finished national or provincial cotton research projects more than 30 items, and
breeding nearly 10 high-yielding, disease-resistant and high-quality cotton varieties, such as Jimian169, JiH 170, Ji178 etc.
Fig.5. Expression patterns of GbERFb
in Sea island cotton. A the effect of
exogenous hormones treatments with
ET, MeJA and SA in leaves, B the effect
of exogenous H2O2 and wounding
in leaves, C the effect of inoculating
Verticillium dahliae in leaves and roots.
Data are averages of three replicates
by mean ±SD
Fig.8. Response of GbERFb transgenic tobacco plants to Verticillium wilt. The tobacco leaves were collected from non-transgenic
tobacco line and three transgenic tobacco lines (L1-L3) of 4 weeks and inoculated with Verticillium wilt. The non-transgenic tobacco
line acted as the CK.
Fig.6. Analysis of the GbERFb transcriptional
activation activity with the GCC sequence by
yeast one hybrid.A: Diagram of the reporters,
effectors construct used in the assays. PCYC1
the promoter of the minimal promoter of the
yeast iso-1-cytochrome C gene, PGAL1 the
promoter of galactose-inducible expression of
gene, CYC1 terminater the terminater of the
Cytochrome transcription gene. B: Comparison
of the transactivation activity of GbERFb and
other control by lifted filter in v yeast cells. 1
pLacZi /pYES3, 2 pLacZi/pYES3-GbERFb,
3p3×mGCC-LacZi/pYES3, 4 p3×mGCC-LacZi/
pYES3-GbERFb, 5 p3×GCC-LacZi/pYES3, 6
p3×GCC-LacZi /pYES3-GbERFb.
Fig.7. Interaction between the GbERFb and
the candidate protein GbMAPKb by yeast two
hybrid. The competent cell AH109 harbored
the Gal4 DNA-binding domain (BD) and Gal4
activation domain (AD) fusion constructions.
The pGbERFb-GADT7/pGbMAPKb-GBKT7
and other control were selected on SD
medium lacking Leu and Trp (DDO) and
interaction was selected on SD medium
lacking Leu, Trp, His and Ade (QDO) for 5
days. All medium contained the X-a-gal.