11 / 23
Page 109
Notes:
conferenceseries
.com
Volume 11
Journal of Proteomics & Bioinformatics
ISSN: 0974-276X
Structural Biology 2018
September 24-26, 2018
September 24-26, 2018 | Berlin, Germany
14
th
International Conference on
Structural Biology
New steroid based amphiphiles for membrane protein structural study
Muhammad Ehsan
and
Pil Seok Chae
Hanyang University, South Korea
B
iological membranes contain a variety of membrane proteins that act as receptors, signal transducers, channels, transporters,
motors and anchors. High resolution structures of membrane proteins are of paramount importance for understanding
mechanism of action at a molecular level and are of significant implications for biomedical and pharmaceutical applications.
Detergents, amphipathic molecules, serve as indispensable tools for extracting membrane proteins from the membranes
and maintaining them in a soluble and active state, which is essential for successful downstream characterizations. However,
membrane proteins in a conventional detergent tend to aggregate and denature over time. To resolve an issue associated with
membrane protein stability, we developed steroidal amphiphiles with a penta-saccharide head group. When these agents were
evaluated for the ability to stabilize a diverse range of membrane proteins, some representatives conferred marked stability to
membrane proteins tested here compared to a conventional detergent.
Muhammad Ehsan et al., J Proteomics Bioinform 2018, Volume 11
DOI: 10.4172/0974-276X-C2-116