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Volume 11
Journal of Proteomics & Bioinformatics
ISSN: 0974-276X
Structural Biology 2018
September 24-26, 2018
September 24-26, 2018 | Berlin, Germany
14
th
International Conference on
Structural Biology
Vitamin E-based glycoside amphiphiles for membrane protein structural studies
Lubna Ghani, Muhammad Ehsan
and
Pil Seok Chae
Hanyang University, South Korea
M
embrane proteins play crucial roles in regulating cellular functions at the interface between cells and their environments
or on the surface of organelles. The major bottleneck for their structure study arises from their instability outside the
native environments. Amphipathic agents called detergents, form micelles which mimic the lipid environment and are widely
used for protein structural and functional studies. However, conventional detergents such as DDM and OG have limitations in
maintaining the solubility and stability of membrane proteins in an aqueous solution. In this study, we introduced a novel class
of amphiphiles consisting of vitamin E and saccharide units as the hydrophobic and hydrophilic groups, respectively. These
agents showed remarkable efficacy toward stabilization and visualization of membrane protein complex, allowing us to clearly
visualize a challenging membrane protein complex using electron microscopy.
Biography
Lubna Ghani has completed her Master’s in Chemistry from Pakistan. Currently she is pursuing her PhD with Professor Pil Seok Chae in Bionano Engineering from
Hanyang University, South Korea. Her research interest lies in development of novel amphipathic agents for membrane protein structure studies.
Lubna Ghani et al., J Proteomics Bioinform 2018, Volume 11
DOI: 10.4172/0974-276X-C2-116