traditional-medicine-2016_posters-accepted-abstracts

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Volume 5, Issue 2 (Suppl) J Tradi Med Clin Natur
ISSN: JTMCN, an open access journal
Page 77
Notes:
Traditional Medicine 2016
September 14-16, 2016
conferenceseries.com
September 14-16, 2016 Amsterdam, Netherlands
6th International Conference and Exhibition on
Traditional & Alternative Medicine
Szu-Ying Wu et al., J Tradi Med Clin Natur 2016, 5:2 (Suppl)
Effects of Toona sinensis leaf extracts on cytokine levels in RAW264.7 cells treated with LPS
Szu-Ying Wu, Chia-Chun Shih, Shih-Meng Tsai and Li-Yu Tsai
Kaohsiung Chang Gung Memorial Hospital, Taiwan
Background: Sepsis remains a major cause of mortality in intensive care. Previous studies have pointed out that sepsis is caused by
excessive inflammatory responses, leading to cell and organ damage in a host. In addition, Toona sinensis is believed to possess anti-
oxidant and anti-inflammatory properties.
Objectives: To evaluate the effects of Toona sinensis leaf extracts (TS) on the cytokine levels in the RAW264.7 cells treated with LPS.
Methods: RAW264.7 macrophages were treated with TS extracts (25, 50, 75 µg/mL) for 2 hours before the treatment of LPS (1 µg/
ml) for 24 hours. Cytokines in the supernatants of cells were determined by Bio-Plex Pro mouse cytokine assay kit for IL-6, IL-10, and
TNF-α, which IL-6 and TNF-α were proinflammatory and IL-10 was anti-inflammatory cytokines.
Results: Compared with those in the control group, the levels of IL-6, TNF-α, and IL-10 were increased in the LPS group without
TS. However, the levels of IL-6 and TNF-α were significantly decreased in the LPS group with TS compared with those without TS.
Discussion: Our results indicated that the TS pretreatment could decrease the levels of IL-6 and TNF-α in the RAW264.7 cells treated
with LPS. This finding was consistent with the report of Chao-ChuanWang et al., who had proposed that the TS extracts could inhibit
the level of TNF-α in microglia-mediated neuroinflammation by LPS. Additionally, HL Yang et al., demonstrated that the TS could
suppress LPS-induced inflammation and migration in vascular smooth muscle cells via NF-κ B activation. Thus, we suggested that the
TS might decrease the levels of TNF-α and IL-6 via inhibition of NF-κB pathway in RAW264.7 cells treated with LPS.
Conclusions: In summary, TS may be a potentially effective adjuvant therapy to LPS-induced sepsis.
Biography
Szu-Ying Wu has completed his Undergraduate studies at Chang Gung University and earned his Medical degree in 2012. He is a CAM resident at Kaohsiung
Chang Gung Memorial Hospital with a major in Acupuncture and Traumatology. He is the Vice Secretary-general of Taiwan Association of Chinese Medicine and
Pharmacy Quality. His research topics focus on low level laser acupuncture and treatment of sleep disorders. His recent clinical interest is Sports Medicine.