Volume 6, Issue 4(Suppl)
Transl Med 2016
ISSN: 2161-1025, an open access journal
Translation Medicine & World Oncologists 2016
November 28-30, 2016
Translational Medicine and Oncologists Meet
November 28-30, 2016 San Francisco, USA
Annual Conference on
Activated hepatic stellate cells promote angiogenesis via interleukin-8 in hepatocellular carcinoma
The first Affiliated Hospital of Bengbu Medical College, China
Chemokines have been recognized as important modulators of angiogenesis and they play critical roles in the
development and metastasis of hepatocellular carcinoma (HCC), although their origins and latent molecular mechanisms remain
The aim of this study was to investigate how activated hepatic stellate cells (a-HSCs) promote angiogenesis in HCC.
A total of 22 HCC patients were enrolled randomly. We used immunohistochemistry; western blotting and enzyme-linked
immunosorbent assay (ELISA) to analyze the production of interleukin-8 (IL-8) in a-HSCs derived fromHCC tissues. The angiogenic
effects of IL-8 in vitro and in vivo were assessed by ELISA, real-time quantitative polymerase chain reaction, capillary tube formation
assay and chick embryo chorioallantoic membrane assay.
The present study showed that IL-8 was enriched predominantly in the tumor stroma of HCC tissues and was mainly
derived from a-HSCs, rather than from hepatoma cells, in vivo and in vitro. Angiogenesis was most active at the invading edge, which
was close to the a-HSCs. The angiogenic effect was dramatically attenuated by an IL-8 neutralizing antibody both in vitro and in
vivo. Moreover, the IL-8 neutralizing antibody down-regulated Ser727-phosphorylated STAT3 levels in hepatoma cells treated with
a-HSCs conditioned medium.
These findings reveal that a-HSCs within the stroma of HCC contribute to tumor angiogenesis via IL-8.
Bing Zhu is a Doctor and has more than 10 years of experience in General Surgery department. He has completed his PhD from Zhongshan University's third affiliated
hospital. Currently, his major directions of research are Tumor Angiogenesis.[email protected]
Bing Zhu, Transl Med 2016, 6:4(Suppl)http://dx.doi.org/10.4172/2161-1025.C1.020