biochemistry-2016_scientifictracks-abstracts

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Volume 5, Issue 3(Suppl) Biochem Anal Biochem 2016
ISSN: 2161-1009, Biochem an open access journal
Page 23
Notes:
Biochemistry 2016
October 10-12, 2016
conferenceseries.com
Biochemistry
October 10-12, 2016 Kuala Lumpur, Malaysia
International Conference on
Effects of lipoic acid supplementation on activities of cyclooxygenases and levels of prostaglandins E2
and F2 alpha metabolites, in the offspring of rats with Streptozotocin-induced diabetes
Hisham Y Al-Matubsi1, Ghaleb A Oriquat2, Mahmood Abu-Samak3, Othman A Al Hanbali4 and Maher D Salim5
1University of Petra, Jordan
2Al-Ahliyya Amman University, Jordan
3Applied Science University, Jordan
4Applied Science University, Jordan
5Middle East University, Jordan
Background & Aim: Uncontrolled diabetes mellitus (DM) is an etiological factor for recurrent pregnancy loss and major congenital
malformations in the offspring. Antioxidant therapy has been advocated to overcome the oxidant-antioxidant disequilibrium inherent
in diabetes. Our aims were to evaluate the protective effect of lipoic acid (LA) on fetal outcome and to elucidate changes that may be
involved in the mechanisms implicit diabetic fetopathy.
Methods:Female rats were rendered hyperglycemic using streptozotocin and thenmated with normal male rat. Pregnant non-diabetic
(group-1; n=9 and group-2; n=7) or pregnant diabetic (group-3; n=10 and group-4; n=8) rats were treated daily with either LA (30
mg/kg body weight; groups-2 and 4) or vehicle (groups-1 and 3) between gestational days 0 and 15. On day 15 of gestation, the rats
were sacrificed and the fetuses, placentas and membranes dissected out of the uterine horns. Following morphological examination,
the fetuses, placentas and membranes were homogenized and used to measure cyclooxygenase (COX) activities and metabolisms of
prostaglandin (PG) E2 (PGEM) and PGF2α (PGFM) levels. Maternal liver and plasma and in the fetuses of all groups..
Results: Supplementation of diabetic rats with LA was found to significantly (p<0.05) reduced resorption rates in diabetic rats and
increased mean fetal weight compared to vehicle-treated diabetic (V-TD) group. Treatment of diabetic rats with LA (LA-TD) leads
to a significant (p<0.05) increase in liver and plasma total glutathione, in comparison with V-TD rats. Decreased levels of PGEM and
elevated levels of PGFM in the fetuses, placentas and membranes were characteristic of experimental diabetic gestation associated
with malformation. LA treatment to diabetic mothers failed to normalize levels of PGEM to the vehicle-treated control rats. However,
the levels of PGEM in malformed fetuses from LA-TD mothers was significantly (p<0.05) higher than those in malformed fetuses
from V-TD rats.
Conclusions: LA can reduce congenital malformations in the offspring of diabetic rats at day 15 of gestation. Thus, LA treatment did
not completely prevent the occurrence of malformations, other factors such as arachidonic acid deficiency and altered prostaglandin
metabolism may be involved in the pathogenesis of the diabetes-induced congenital malformations.
Biography
Hisham Al-Matubsi has completed his PhD from Victoria University-Australia and worked at different academic levels in different reputable academic organizations such
as Victoria University-Victoria, Australia, Cincinnati University-Ohio, USA and now at The University of Petra-Amman, Jordan. Dr Al-Matubsi’s professional interests are in
the area of reproductive physiology research, with a specific emphasis on ovarian hormones and changes that may be involved in the mechanism(s) underlying diabetic
fetopathy. He has published more than 20 papers in reputed journals and is serving as an editorial board member of repute journal Diabetes and related Disorders.
Hisham Y Al-Matubsi et al., Biochem Anal Biochem 2016, 5:3(Suppl)