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Chromatography 2016
September 21-23, 2016
Volume 7, Issue 5(Suppl) J Chromatogr Sep Tech 2016
ISSN: 2157-7064 JCGST, an open access journal
conferenceseries .com
September 21-23, 2016 Amsterdam, Netherlands
World Congress on Chromatography
A novel toolbox with chromatogram fingerprints for lysis monitoring in E.coli bioprocesses
Vignesh Rajamanickam, David Wurm, Christoph Herwig and Oliver Spadiut
Vienna University of Technology, Austria
The bacteriumEscherichia coli is a well-studied recombinant host organismwith a plethora of applications in biotechnology.
High valuable biopharmaceuticals, such as antibody fragments and growth factors, are currently being produced in E. coli.
However, the high metabolic burden during recombinant protein production can lead to cell death, consequent lysis and
undesired product loss. Thus, fast and precise analyzers to monitor E. coli bioprocesses and to retrieve key process information,
such as the optimal time point of harvest, are needed. However, such reliable monitoring tools are still scarce to date. In
this study, we cultivated a recombinant E. coli strain producing a recombinant single chain antibody fragment (scFv) in the
cytoplasm. In bioreactor cultivations, we purposely triggered cell lysis by pH ramps. We developed a novel toolbox using UV
chromatogram fingerprints and chemometric techniques to monitor these lysis events and used flow cytometry (FCM) as
reference method to quantify viability offline. Furthermore, we tested the applicability of the novel toolbox for montiroing
other E. coli bioprocesses. We are convinced that this toolbox will not only facilitate E. coli bioprocess monitoring, but will also
allow enhanced process control in the future.
Biography
Vignesh Rajamanickam procured his Master of Science in Pharmaceutical Biotechnology from Hamburg University of Applied Sciences, Germany and, Bachelor
of Technology in Biotechnology from Anna University, India. He started his PhD on March 2014 in Biochemical Engineering from Vienna University of Technology
(VUT), Austria. Currently, he is working as a Project Assistant for developing a novel PAT tool for bioprocess monitoring and control in Christian Doppler laboratory
for mechanistic and physiological methods for improved bioprocesses, VUT, Austria.
Vignesh Rajamanickam et al., J Chromatogr Sep Tech 2016, 7:5(Suppl)