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Chromatography 2016
September 21-23, 2016
Volume 7, Issue 5(Suppl) J Chromatogr Sep Tech 2016
ISSN: 2157-7064 JCGST, an open access journal
conferenceseries .com
September 21-23, 2016 Amsterdam, Netherlands
World Congress on Chromatography
Separation of hydrophobic amino acid enantiomers in CEC system by molecular imprinting technique
Koray Şarkaya1,4, Süleyman Aşır3, Ali Derazhamshir1, Fatma Yılmaz2, Handan Yavuz1 and Adil Denizli1
1Hacettepe University, Turkey
2Abant Izzet Baysal University, Turkey
3Middle East Technical University, Turkey
4Düzce University, Turkey
C apillary electrochromatography (CEC), first described by Pretorius, is a rapidly evolving hybrid technique between CE
and LC. Analytes may be separated in CEC by the combined action of partitioning between stationary phase and mobile
phase (chromatographic interactions) and by their difference in electrophoretic mobility (movement of solutes by electrical
forces). Therefore, CEC usually possess higher efficiency and selectivity as compared with classical CE and LC. The use of CEC
for chiral analysis has become popular in recent years due to high separation efficiency. Separation of enantiomers is very
important because they may show different biological activities. Many single enantiomers of amino acids were also used for
the synthesis of biologically important compounds. In this work, enantiomeric separation by CEC was extensively carried out
using chiral monolithic capillary column. In this presentation, we will describe about the monolithic column, which is based
on N-methacryloyl-(L)-phenylalanine methyl ester (MAPA) as hydrophobic monomer in CEC system.
Biography
Koray Şarkaya is PhD student from Hacettepe University and has done his PhD studies from Biochromatography and Biodiagnostics Research Group of Hacettepe
University. He has been a Research Assistant at the Düzce University since 2009.
Koray Şarkaya et al., J Chromatogr Sep Tech 2016, 7:5(Suppl)