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Chromatography 2016
September 21-23, 2016
Volume 7, Issue 5(Suppl) J Chromatogr Sep Tech 2016
ISSN: 2157-7064 JCGST, an open access journal
conferenceseries .com
September 21-23, 2016 Amsterdam, Netherlands
World Congress on Chromatography
Dendron-based stationary phases for hydrophobic interaction chromatography
Sena Yaman1,2, Marco A Mata-Gómez1, José González-Valdez1, Jesús A Valencia-Gallegos1, Marco Rito-Palomares1 and Canan Tari2
1Tecnologico de Monterrey, México
2Izmir Institute of Technology, Turkey
Hydrophobic Interaction Chromatography (HIC) is a powerful technique used to purify proteins. It relies on the interaction
between non-polar groups on the hydrophobic patches of the proteins and ligands on the HIC medium. This interaction
varies according to the hydrophobicity of biomolecules, leading to the separation of proteins in a complex mixture. In this
work, two novel stationary phases for HIC were synthesized by incorporating dendronitic structures, branched molecules.
The branched nature of the dendrons allowed the attachment of high amount of hydrophobic ligands to the support material.
Depending on the number of branches of the dendron used, ligand densities were calculated as 82.5±11 and 175.6±5.7 μmol
ligand/ml resin for Sorbent 1 and Sorbent 2, respectively. UV-Vis absorption spectra of the modified sorbents exhibited a band
at 287 nm corresponding to the aromatic ring present on the dendrons suggesting their incorporation onto the sorbents. FTIR
analysis evidenced the aromatic and carbonyl groups suggesting the presecence of dendrons with hidrophobic ligands on the
sorbents. Adsorption capacity of the sorbents was evaluated in static and dynamic mode using bovine serum albumin (BSA)
under high concentrations of ammonium sulfate (AS). Increasing AS concentration from 1.5-2 M led to significant increases in
adsorption capacity. Dynamic adsorption was influenced by flow velocity. This innovative design allowed to increase the ligand
density and therefore the adsorption capacity of the sorbents. This technology may permit to reduce the amount of sorbent to
be used in a bioprocess and thus use smaller columns resulting in faster chromatoraphic processes.
Biography
Sena Yaman has completed her MSc degree in Middle East Technical University and is currently working on her Doctoral studies in Izmir Institute of Technology,
Department of Bioengineering.
Sena Yaman et al., J Chromatogr Sep Tech 2016, 7:5(Suppl)