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.com
Volume 8, Issue 5 (Suppl)
J Chromatogr Sep Tech, an open access journal
ISSN: 2157-7064
Chromatography 2017
August 07-09, 2017
August 07-09, 2017 | Rome, Italy
4
th
World Congress on
Chromatography
Application of combined chromatographic techniques in the screening and separation of complex
triterpenoid saponins from
Pithecellobium saman
Maria de Fátima Simão Jucá Cruz, Gabriela M Pereira, Bernadete P da Silva
and
José P Parente
Institute of Research on Natural Products Walter Mors, Brazil
Introduction & Aim:
Pithecellobium saman
(Leguminosae) is native to tropical America, nowadays widely cultivated and
naturalized through tropical regions with ornamental purposes. The leaves are livestock forage supplement and this species
is used in traditional medicine as a remedy for the treatment of different diseases. Nonetheless, no chemical investigations or
biological evaluations were carried out on the constituents of this species. According to the literature, complex triterpenoid
saponins are shown to possess several physiological properties depending on their amphipathic chemical structures, such
as the capacity for alteration of membrane permeability. Additionally, these compounds have been reported to possess
therapeutic potential for immune system modulation through different mechanisms. As part of our ongoing efforts in
discovering potentially bioactive compounds from natural sources, the present study is concerned with the combination of
different chromatographic methods to improve the isolation of individual substances from a complex mixture of compounds
with similar chemical structure.
Methodology:
The root barks of
Pithecellobium saman
were extracted with MeOH. After concentration, the resulting
residue was partitioned between water and n-BuOH. The organic phase was concentrated, submitted to molecular exclusion
chromatography and monitored by thin layer chromatography, affording four fractions containing a complex mixture
of saponins. The fractions were submitted to reversed phase high performance liquid chromatographic analysis, using an
ultraviolet detector, with MeOH:H2O (65:35) as solvent system, a flow rate of 0.5 mL and a chromatographic run time of 45
minutes.
Findings:
By a combination of chromatographic methods and spectroscopic techniques, it was demonstrated that the fractions
were composed of a multicomponent mixture of complex triterpenoid saponins in different proportions.
Conclusion:
A combination of different chromatographic techniques was required to perform the isolation of the substances
from a complex mixture and to establish a preliminary structural characterization of its constituents.
Biography
Maria de Fátima Simão Jucá Cruz completed her Graduation in Chemistry and Master’s Degree at Federal Rural University of Rio de Janeiro (UFRRJ). She is
pursuing her Doctorate degree in Natural Products Chemistry at UFRJ in Brazil.
fatima_ufrrj@hotmail.comMaria de Fátima Simão Jucá Cruz et al., J Chromatogr Sep Tech 2017, 8:5(Suppl)
DOI: 10.4172/2157-7064-C1-032