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.com
Volume 8, Issue 5 (Suppl)
J Chromatogr Sep Tech, an open access journal
ISSN: 2157-7064
Chromatography 2017
August 07-09, 2017
August 07-09, 2017 | Rome, Italy
4
th
World Congress on
Chromatography
Quantification of acetamiprid and thiacloprid residues in field-incurred butterbur samples using
liquid chromatography-tandem mass spectrometry
Han Sol Lee, Hyung Suk Chung, Md Humayun Kabir, Md Musfiqur Rahman
and
Jae-Han Shim
Chonnam National University, South Korea
A
n analytical method was developed to quantify the residual levels of the neonicotinoid insecticides acetamiprid and
thiacloprid in field-incurred butterbur samples using liquid chromatography-tandem mass spectrometry. Samples were
extractedwith acetonitrile andpartitionedwithdichloromethane. After partitioning, purificationwas conductedusing a Florisil®
cartridge. Linearity of the two compounds over a concentration range of 0.004–0.4 μg/mL was excellent, with determination
coefficients (R2)≥0.9998. The limits of detection (LOD) and quantitation (LOQ) for both acetamiprid and thiacloprid were
0.0006 and 0.002 mg/kg, respectively. The average recoveries for acetamiprid and thiacloprid at two spiking levels (0.02 and 0.1
mg/kg, i.e., 10×LOQ and 50×LOQ) were between 78.23 and 82.15%, with relative standard deviations≤7.22%. The method was
successfully applied to field-incurred samples treated with a commercial pesticide product, either once (zero or 7 days before
harvest) or twice (0 and 7, 7 and 14, or 14 and 21 days before harvest). The highest and lowest residues were obtained for the
0 and 7 days’ treatment and the 14 and 21 days’ treatment, respectively. The developed method is simple and accurate and can
be extrapolated to other leafy vegetables.
Biography
Han Sol Lee is pursuing her Master degree at Chonnam National University, South Korea. She is interested in agricultural food safety from harmful materials such
as pesticides and veterinary antibiotics. She participated in many projects that developed the analytical method for determining pesticides and veterinary antibiotics
in several environmental factors such as crops, water, and monitoring.
fjqngksthf@naver.comHan Sol Lee et al., J Chromatogr Sep Tech 2017, 8:5(Suppl)
DOI: 10.4172/2157-7064-C1-032