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Journal of Clinical & Experimental Pharmacology | ISSN: 2161-1459 | Volume: 8
International Conference on
July 18-19, 2018 | Atlanta, USA
Pharmacology and Ethnopharmacology
11
th
International Conference and Exhibition on
Pharmaceutical Oncology
&
Baicalin administration has a protective effect on Hyperglycemia-induced malformation of
cardiovascular system
Jian-xin Liang, Lin-rui Gao, Li-guo Chen, Guang Wang
and
Xue-song Yang
Jinan University, China
Aims:
Baicalin is a traditional Chinese medicine for tocolysis. Whether it can protects early embryonic cardiovascular
development caused by gestational diabetes is obscure, and the mechanism remains unclear. In this research, early chicken
embryo was used as a model to explore the molecular mechanismof baicalin in reducing the early cardiovascular developmental
deformity caused by high glucose environment.
Methods:
We found that 6μM baicalin administration can attenuate the death rate and retardation of chicken embryos caused
by high glucose environment significantly. Thus, we observed the chick embryos in HH7, HH8, HH10, HH11stages, which
are treated with simple saline, high glucose (50 mM) and/or Baicalin (6 μM). In this study, we used immunofluorescence,
situ hybridization, RT-PCR, Western blot, qPCR and others to observe the expression of the key transcription factors, and
the changes of autophagy-related genes, apoptosis-related genes, ROS in the development of cardiovascular so that to study
whether or not Baicalin could attenuate hyperglycemia-induced malformation of cardiovascular system and the mechanism. At
the same time, we studied the mechanism involving ROS, autophagy and apoptosis, combined with HUVEC cell. In addition,
we also used Baicalin to treat the mice in diabetes model induced by Streptozotocin(STZ) and observed whether or not this has
the protective impact on the blood glucose and other organs of diabetes mellitus mice.
Results:
Hyperglycemia-enhanced cell apoptosis was reduced in embryos and HUVECs in the presence of Baicalin.
Hyperglycemia-induced excessive ROS production was inhibited when Baicalin was administered. Analyses of classical
antioxidant enzymes, MQAE and GABAA suggested Baicalin plays an antioxidant role in chick embryos possibly through
suppression of outwardly rectifying Cl(-) in the high-glucose microenvironment. What’s more, hyperglycemia-enhanced
autophagy fell in the treatment of Baicalin, through affecting the ubiquitin of p62 and accelerating autophagy flux. Both
Baicalin and Vitamin C could reduce apoptosis, but CQ did not, suggesting autophagy to be a protective function on the cell
survival. In mice, Baicalin decreased the elevated blood glucose level caused by STZ.
Conclusions:
In brief, these data suggest that hyperglycemia-induced embryonic cardiovascular malformation can be
attenuated by Baicalin administration through suppressing the excessive production of ROS and autophagy. Baicalin could be
a potential candidate drug for women suffering from gestational diabetes mellitus.
Biography
Jian-Xin Liang has completed his undergraduate course from the Hubei University of Chinese Medicine. Now she is completing her master study in Jinan University
School of Medicine. She has published 2 papers, including “Atg7-mediated autophagy is involved in the neural crest cell generation in chick embryo” Molecular
Neurobiology, and “BRE modulates granulosa cell death to affect ovarian follicle development and atresia in the mouse.” Nearly for 2 years, she has been doing
research about the impact of baicalin on the heart and vascular development of embryos, which are in the PGDM (previous gestational mellitus) environment.
545054487@qq.comJian-xin Liang et al., Clin Exp Pharmacol 2018, Volume: 8
DOI: 10.4172/2161-1459-C1-029